苦参碱对胰腺癌吉西他滨耐药及Aurora—A表达的影响

目的探讨苦参碱对胰腺癌吉西他滨耐药及Aurora—A表达的影响。方法不同浓度苦参碱联合吉西他滨处理PANC-1及其耐药细胞株PANC-1／R2，流式细胞技术检测细胞株凋亡情况；qPCR法测定Aurora-A mRNA表达。结果流式细胞技术显示苦参碱和吉西他滨不同给药方式在PANC-1组和合PANC—1／R2组内凋亡率差异无统计学意义，但两组间比较差异有统计学意义。qPCR结果，PANC—1组在空白对照、吉西他滨（400μg／ml）、苦参碱（8mg／ml）及吉西他滨（400gg／ml）联合苦参碱（8μg／ml）处理后，Aurora—AmRNA表达分别是（1．001±0．041），（0．884±0．063），（0．884±0．063）和（0．957±0．084），与对照纽比较差异均无统计学意义（P〉0．05）；PANC—1／R2组分别是（1．835±0．072），（1．761±0．052），（1．879±0．068）和（1．910±0．086），与对照组比较差异无统计学意义（P〉0．05）。结论苦参碱与吉西他滨联合作用具有诱导胰腺癌细胞凋亡的抗肿瘤作用，但不能逆转胰腺癌吉西他滨耐药的作用，不能增加化疗敏感性，也不具有调节Aurora-A表达的作用。提示苦参碱可通过诱导凋亡途径发挥抗胰腺癌作用，但其作用不十分显著。

Objective To evaluate the effect of matrine on inducing apoptosis of pancreatic cancer cells, and regulating the expression of Aurora-A and reversing gemcitabine-resistance of pancreatic cancer. Methods Apoptosis of PANC-1 and PANC-1/R2 cells were analyzed by Flow Cytometry after treatment of matrine and gemcitabine. Aurora-A gene expression of PANC-1 / and PANC-1/R2 were analyzed by qPCR after treatment of matrine and gemcitabine. Results The outcome of Flow cytometry shows that apoptosis rates of pancreatic cells had no significant difference in groups but significant difference between the PANC-1 and PANC-1/R2 groups. The same phenomenon was found of Aurora-A mRNA expression in the two groups. Conclusions The combination of matrine andh gemcitabine can induce apoptosis of pancreatic cancer cells, but can not reverse the effects of gemcitabine-resistance of pancreatic cancer, can not increase sensitivity to chemotherapy, also has no impact on expression of Aurora-A. The results indicates that matrine can play a certain role of anti-pancreatic cancer by inducing apoptosis.