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集成化荧光激活液滴分选系统研究

Research on Integrated Fluorescence Activated Droplet Sorting System
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摘要 高通量的荧光激活液滴分选技术在大规模生化试验中能够显著降低实验成本,缩短实验时间,因此在微生物菌株筛选、新药研发、高通量单细胞研究等众多领域具有广泛的应用。然而,目前使用的荧光激活液滴分选系统大多基于光学试验器件搭建,系统的灵活性、稳定性相对较差,搭建的技术门槛较高,限制了该技术在生命科学研究等领域的推广应用。设计一种集成化的荧光激活液滴分选系统,通过特定光路设计,系统所需的荧光激发和探测光路实现集成化、模块化封装,荧光激发和探测功能模块缩小到160 mm×143 mm×54 mm,大幅缩减系统体积。可以通过给普通显微镜增加功能模块的方式,快速实现分选系统的搭建,从而提高荧光激活液滴分选技术的易用性。相比现有文献报道的荧光激活液滴分选系统,成本下降到1/5,体积减小为1/40,有利于该技术的工业化推广。
出处 《中国生物医学工程学报》 CAS CSCD 北大核心 2016年第3期370-374,共5页 Chinese Journal of Biomedical Engineering
关键词 荧光激活 液滴分选 集成化 fluorescence activated droplet sorting system integration
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参考文献11

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