HPLC法测定复方氯霉素洗剂中氯霉素和水杨酸的含量

Determination of the Content of Chloramphenicol and Salicylic acid in Compound Chloramphenicol Lotion by HPLC

目的建立测定复方氯霉素洗剂中氯霉素和水杨酸含量的方法。方法采用高效液相色谱法,Kromasil C18柱（4.5×250mm,5μm）;流动相：0.01mol·L-1庚烷磺酸钠缓冲液（磷酸调节p H值至2.90）-甲醇（68∶32）;检测波长：277nm;流速：1.0m L·min-1。结果氯霉素在20-200μg·m L-1、水杨酸在40-400μg·m L-1范围内呈良好的线性关系,y=34.573x＋8.0449,r=0.9999;y=11.215x＋10.606,r=0.9999。回收率分别为100.03%（RSD=1.08%,n=9）、100.26%（RSD=1.32%,n=9）。结论本方法快速、准确、重现性良好,可用于该洗剂的含量测定。

OBJECTIVE To establish the quantitative determination method of the contents of chloramphenicol and salicylic acid in Compound Chloramphenicol Lotion. METHODS Kromasil C18column（ 4. 5 × 250 mm,5μm）,mobile phase： 0. 01mol·L- 1Sodium 1-heptanesulfonate（ Adjusts the p H to 2. 90 with the Phosphoric acid）-Methanol（ 68 ∶ 32）,detection wavelength： 277 nm,flow rate： 1. 0m L · min- 1. RESULTS Chloramphenicol and Salicylic acid was linear a range of 20 - 200μg·m L- 1and 40 - 400μg·m L- 1,linear equation was y = 34. 573 x ＋ 8. 0449,r= 0. 9999 and y = 11. 215 x ＋ 10. 606,r = 0. 9999,the average recovery was 100. 03%（ RSD = 1. 08%,n = 9） and100. 26%（ RSD = 1. 32%,n = 9）. CONCLUSION This method is rapid,accurate,reproducible,and can be used to assay the content of this lotion.