摘要
建立了梯度洗脱HPLC法测定环丙氨嗪有关物质。采用Agilent Eclipse plus C 18色谱柱(250 mm×4.6 mm,5μm),流动相A为0.02 mol/L醋酸铵溶液,流动相B为甲醇,线性梯度洗脱,流速1.0 m L/min,检测波长为213 nm。试验结果显示,环丙氨嗪与已知5种杂质峰完全分离。本方法简单灵敏,可用于环丙氨嗪中有关物质的检测。
To develop a gradient HPLC method for the determination of related substance in cyromazine,the chromatographic column was Agilent Eclipse plus C18（ 250 mm × 4. 6 mm,5 μm） was used,the mobile phase consisted of 0. 02 mol / L ammonium acetate（ A） and methanol（ B） with gradient program. The flow rate was 1. 0m L / min. The detection wavelength was at 213 nm. The results showed good separation of cyromazine from five impurities. The method is simple and sensitive for the impurity detection of cyromazine.
出处
《中国兽药杂志》
北大核心
2016年第6期48-51,共4页
Chinese Journal of Veterinary Drug
