摘要
建立了反向高效液相色谱法检测四味穿心莲散中穿心莲内酯和脱水穿心莲内酯含量的方法。试验采用十八烷基硅烷键合硅胶色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.2%磷酸溶液梯度洗脱,流速为1.0 m L/min,柱温30℃;穿心莲内酯检测波长226 nm,脱水穿心莲内酯检测波长252 nm;穿心莲内酯和脱水穿心莲内酯的含量测定范围分别为2.32~37.14μg/m L（r=1.0000）,2.00~31.94μg/m L（r=1.0000）;加样平均回收率分别为99.12%、98.84%;RSD分别为0.60%、0.62%;该方法方便、准确、可靠,适用于测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量。
To establish a RP- HPLC method for determination of andrographolide and ehydroandrographolide in Siwei Chuanxinlian San,analysis was performed on a C18 column（ 250 mm × 4. 6 mm,5 μm） with a gradient elution mode of methanol- 0. 2% phosphoric acid at a flow rate of 1. 0 m L / min and a column temperature of30 ℃. The determination wave length of andrographolide was 226 nm while dehydroandrographolide was 252 nm.The linear range was 2. 32 ~ 37. 14 μg / m L（ r = 1. 0000） and 2. 00 ~ 31. 94μg / m L（ r = 1. 0000）,and the average recovery was 99. 12% and 98. 84% with RSD of 0. 60% and 0. 62%,respectively. This method was convenient,accurate and reliable for the determination of andrographolide anddehydroandrographolide in Siwei Chuanxinlian San.
出处
《中国兽药杂志》
北大核心
2016年第6期56-60,共5页
Chinese Journal of Veterinary Drug
