水稻恶苗病菌对咪鲜胺的敏感性及生物学特性的研究

Research on Sensitivity and Biological Characteristics of Fusarium fujikuroi to Prochloraz

为了明确黑龙江省水稻恶苗病菌对咪鲜胺的产生抗性情况,利用菌丝生长速率法检测了来自黑龙江省8个县(市)的32个恶苗病菌株对咪鲜胺的敏感性,确定敏感基线值为0.068 9μg·m L-1,有15.63%供试菌株为低抗菌株,3.12%为中抗菌株,没有检测到高抗菌株,表明黑龙江省水稻恶苗病菌对咪鲜胺的敏感性明显下降,已经产生抗性菌株。比较了抗性菌株JMS-3和敏感菌株NH-1的生物学特性,发现抗性菌株在生物学特性方面发生了一定变化,抗性菌株JMS-3菌丝生长速度明显低于敏感菌株NH-1,敏感菌株NH-1最适pH为7,抗性菌株JMS-3最适pH为6,敏感菌株NH-1最适氮源为硝酸钠,抗性菌株JMS-3最适氮源为硝酸钾。

In order to clarify Fusarium fujikuroi resistant to prochloraz,the mycelium growth rate method was used to test sensitivity of 32 Fusarium fujikuroi isolates to prochloraz that collected from 8 countries（cities） in Heilongjiang province,and the sensitive baseline value was 0.068 9 μg·m L^（-1）. The low level resistant strains and the medium level resistant strains accounted for 15.63% and3.12%,respectively,and the high level resistant strains was not detected. The results showed that the sensitivity of Fusarium fujikuroi to prochloraz in Heilongjiang province was significantly lower and resistant strains was produced. Compared the biological characteristics of resistant strains JMS-3 and sensitive strains NH-1,the some changes had taken place in the biological characteristics of resistant strains,and resistant strains JMS-3 mycelium growth rate was significantly lower than sensitive strains NH-1. The optimal pH of sensitive strains NH-1 was 7 and the optimal p H of resistant strains JMS-3 was 6. The optimal nitrogen sources for sensitive strains NH-1 was NaNO_3 and the optimal nitrogen sources for resistant strains JMS-3 was KNO_3.