大鼠肾脏在体持续灌注模型的建立

Establishment of rat models with in situ continuous perfusion of kidney

目的建立大鼠肾脏在体持续灌注模型,为移植肾持续灌注保存的基础研究提供简便可靠的动物模型。方法雌性大鼠30只,根据灌注入口不同随机分为3组:右肾动脉入口组(无需显微缝合)、腹主动脉入口组(需要在显微镜下行腹主动脉穿刺口的缝合)、左肾动脉入口双肾对照组(需要显微缝合左肾动脉开口,在不影响全身循环的情况下实现自身左右肾脏不同保存方法的对照)各10只,均以卵巢静脉作为灌注液的流出道。分别计算3组的灌注成功率与肾脏持续灌注2 h再灌注成功率。结果右肾动脉入口组灌注成功率为80%,再灌注成功率100%;腹主动脉入口组灌注成功率为100%,再灌注成功率为80%;左肾动脉入口双肾对照组灌注成功率为80%,再灌注成功率为50%。结论 3种简便大鼠肾脏在体持续灌注模型均建立成功,对于显微外科初学者,无需显微缝合的右肾动脉入口方法最为简便可靠,次要选择腹主动脉入口组;具有扎实显微外科基础的术者,左肾动脉入口双肾对照组可实现右侧肾脏静态低温保存与左侧肾脏持续灌注保存的自身对照,不失为较好的选择。

Objective To explore an approach for establishing rat models with in situ continuous renal perfusion,providing a convenient and reliable animal model for fundamental research of renal transplantation. Methods According to the perfusion access,30 female rats were randomly divided into three groups. In the right renal artery access group（ n =10）,wound suture under microscope was not required. In the abdominal aorta access group（ n = 10）,the wound of abdominal aorta puncture was sutured under microscope. In the control group（ n = 10）,the opening of the left renal artery was sutured under microscope,and bilateral kidneys were restored by different methods without affecting systemic circulation.The ovarian vein was utilized as the perfusion output. The success rates of continuous kidney perfusion and 2 h reperfusion were statistically compared among three groups. Results In the right renal artery access group,the success rate of perfusion was 80% and 100% for 2 h reperfusion. In the abdominal aorta access group,the perfusion success rate achieved 100% and80% for 2 h reperfusion. In the control group,the success rate of perfusion was calculated as 80% and 50% for 2 h reperfusion. Conclusions Three types of rat models with in situ continuous kidney perfusion are successfully established.For microsurgery beginners,the technique of right renal artery access is most simple and reliable,followed by abdominal aorta access approach. For the surgeons with microsurgery skills,static cryopreservation of the right kidney and continuous perfusion preservation of the left kidney are highly recommended.