禾谷炭疽菌效应分子motif序列研究

Study on the Motif of Effector in Colletotrichum Graminicola

禾谷炭疽菌侵染玉米、小麦等粮食作物而引起的炭疽病,给各国农业生产造成了巨大经济损失。植物病原菌可以利用效应分子来操控寄主植物的防卫反应,从而实现其对植物的侵染、定殖和扩展等过程。研究基于前人对禾谷炭疽菌中已报道的177个候选效应分子（CE）的氨基酸数据,利用MEME对全部CE和不同氨基酸类别的CE进行在线motif分析,未获得较满意结果。随后,结合已报道效应分子（E）所具有保守motif的氨基酸位置范围,对82个具有典型特征E的信号肽序列后30~60 aa进行获取,并将信号肽位置大于28、氨基酸数量较小的E进行剔除,最终明确64个E存在着保守的PAAX motif。该研究丰富了真菌保守motif的类型,为进一步开展禾谷炭疽菌效应分子的motif功能研究提供重要的理论指导。

Colletotrichum graminicola can infect corn,wheat and other food crops,causing tremendous economic losses in agricultural production in many countries. Plant pathogen effector can be used to manipulate the host plant defense responses in order to achieve its infestation,colonization and expansion. Firstly,based on previous results that the amino acid data of 177 candidate effectors（ CE） in C. graminicola,the results are not satisfactory with all and different amino acids type of CEs by using MEME online analysis,and then,according to the position range of the conserved motif,30 ~ 60 aa following signal peptide sequence of 82 effectors have obtained,which have the typical character of effector（ E）,and some effectors were removed with the character of signal peptide length is greater than 28,or the number of amino acids is smaller. There is conserved PAAX motif in 64 effectors,which enrich the conservative motif type in fungus and provide strong theoretical support to further clarify the role of motif of effector.