绵萆薢中甾体类成分的分离与鉴定Ⅱ

Isolation and identification of steroidal saponins from Dioscorea spongiosa Ⅱ

目的对绵萆薢的化学成分进行研究。方法将大孔吸附树脂、硅胶、Sephadex LH-20、正相硅胶、ODS等柱色谱与制备型高效液相色谱法相结合进行化合物的分离制备,并通过理化性质与核磁共振波谱数据鉴定化合物的结构。结果从绵萆薢中分离鉴定了12个单体成分,分别鉴定为原薯蓣皂苷（protodioscin,1）、甲基原薯蓣皂苷（methyl protodioscin,2）、原纤细皂苷（protogracillin,3）、甲基原纤细皂苷（methyl protogracillin,4）、伪原薯蓣皂苷（pseudoprotodioscin,5）、伪原纤细皂苷（pseudoprotogracillin,6）、26-O-β-D-glucopyranosyl-3β,23,26-triol-25（R）-furosta-5,20（22）-dien-3-O-α-L-rhamnopyranosyl（1→4）-[α-L-rhamnopyranosyl-（1→2）]-β-D-glucopyranoside（7）、豆甾醇（stigmasterol,8）、β-谷甾醇（β-sitosterol,9）dioscoreavilloside A（10）、绵萆薢苷B（spongioside B,11）及粉背薯蓣G（hypoglaucin G,12）。结论在对绵萆薢的化学成分进行研究的过程中,分离鉴定了12个甾体类化合物。其中化合物5~8和10为首次从该种植物中分离得到,并首次对化合物7的核磁数据进行了报道。

Objective To study the chemical constituents from Dioscorea spongiosa. Methods The compounds were isolated by chromatographies such as D101 resin,silica gel,Sephadex LH-20,ODS and HPLC,and identified by the physicochemical property and spectral data. Results Twelve steroids were isolated and identified as protodioscin（ 1）,methyl protodioscin（ 2）,protogracillin（ 3）,methyl protogracillin（ 4）,pseudoprotodioscin（ 5）,pseudoprotogracillin（ 6）,26-O-β-D-glucopyranosyl-3β,23,26-triol-25（ R）-furosta-5,20（ 22）-dien-3-O-α-L-rhamnopyranosyl（ 1 → 4）-[α-L-rhamnopyranosyl-（ 1 → 2） ]-β-D-glucopyranoside（ 7）,stigmosterol（ 8）,β-sitosterol（ 9）,dioscoreaviuoside A（ 10）,spongioside B（ 11） and hypoglaucin G（ 12）,respectively. Conclusions Compounds 5- 8 and 10 were obtained from D. spongiosa for the first time,and the1H-NMR,13C-NMR data of 7 was firstly reported in this paper.