不同家族史鼻咽癌患者癌组织Prohibitin的表达

The expression of Prohibitin in patients with nasopharyngeal carcinoma(NPC) in different family history

目的对比Prohibitin(PMB)在不同家族史的鼻咽癌患者癌组织中的表达,以探究鼻咽组织中Prohibitin是否可以作为鼻咽癌潜在标记物。方法收集高癌家系气虚质鼻咽癌患者、鼻咽黏膜慢性炎症患者的鼻咽组织各9例,散发气虚质鼻咽癌患者的鼻咽组织12例,应用实时定量PCR方法检测Prohibitin在各组鼻咽组织中的表达。利用Western-blotting蛋白免疫印迹方法检测Prohibitin在各组患者鼻咽组织中的表达。结果 PHB m RNA在鼻咽黏膜慢性炎症鼻咽组织、散发气虚质鼻咽癌组织、高癌家系气虚质鼻咽癌组织中的2-△△Ct值分别为1.00±0.72、1.08±0.49、0.97±0.41(P>0.05)。PHB蛋白相对表达依次为0.33±0.21、0.59±0.25、1.72±0.96(P<0.05)。分析各组间对比PHB基因表达量无明显变化,但蛋白表达量鼻咽癌患者明显高于正常人,高癌家系鼻咽癌患者蛋白表达量高于散发患者,考虑与级联放大反应有关,有望成为高癌家系气虚质鼻咽癌患者早期诊断及筛查的标志物。

Objective To observe whether Prohibitin（PHB） are differential expression protein in patients with nasopharyngeal carcinoma（NPC） who are Qi-deficiency constitution in familial NPC. To test whether PHB are the key differentially expressed proteins which we have identified previously. Methods It had been collected of 9 cases with Qi deficiency of NPC from familial NPC, 12 case with Qi deficiency of NPC from scattered distributed patients, at the same time collecting 9 cases nasopharyngeal tissue from chronic inflammation of nasopharyngeal mucosa patients, to extract total RNA and protein respectively. The Real Time PCR technology was used to detect the expressions of PHB on the m RNA level and the Western blotting was used to detect the level of PHB. Results the In group of nasopharyngeal tissue from chronic inflammation of nasopharyngeal mucosa patients, Qi deficiency of nasopharyngeal carcinoma from scatteredly distributed patients and high cancer families, PHB m RNA using Real Time PCR showed that 2- △△CT of RTPCR were respectively 1.00 ±0.72,1.08 ±0.49,0.97 ±0.41（P〉0.05）, the PHB protein of Western blot were respectively 0.33 ±0.21、0.59 ±0.25、1.72 ±0.96（P〈0.05）. Conclusions PHB m RNA relative have no obvious changes among three groups, but PHB expression quantity is significantly higher than nasopharyngeal mucosa patients in patients with NPC, and lower than familial NPC patients. Its concern about PHB expression regulation the role in the Ras-driven activation of PI3K/Akt and Raf-1/ERK signaling cascades, PHB can be regarded as a biomarker of NPC.