蜡状芽孢杆菌CLY07菌株的解有机磷特性研究

Study on Organophosphate-solubilizing Characteristics of Bacillus cereus CLY07

通过改良的NBRIP培养基,研究从南方红豆杉根际分离筛选得到的解有机磷蜡状芽孢杆菌CLY07的解磷能力,同时探究培养基中卵磷脂添加量、碳源、氮源、碳氮比及环境条件(培养基p H、温度、装液量及盐度等)对该菌株解磷能力的影响。结果表明:蜡状芽孢杆菌CLY07的解磷效果在改良NBRIP培养基中明显优于蒙金娜有机磷培养基。改良NBRIP培养基以葡萄糖为碳源,硫酸铵为氮源,碳氮比为60∶1,卵磷脂添加量为0.20 g,在其余组分不变条件下,该菌株显示出较高的解磷能力。该菌株最佳的解磷环境条件为培养基p H 7.0,温度30℃,不含Na Cl,装液量为1/5。研究显示与蒙金娜有机磷培养基相比,改良的NBRIP培养基在一定程度上更适合于蜡状芽孢杆菌CLY07解磷特性的研究。此外,培养环境能够影响蜡状芽孢杆菌CLY07的解磷能力。

The characteristics of organophosphatesolubilizing by Bacillus cereus CLY07 isolating from the rhizosphere of Taxus chinensis var. mairei were studied and the modified NBRIP culture medium was used. Moreover, the effects of lecithin content in culture medium, carbon source, nitrogen source, carbon nitrogen ratio and environmental conditions （culture medium pH, temperature, liquid volume and salinity, etc.） on the phosphate solubilizing capacity of Bacillus cereus CLY07 were explored. The result was that the phosphate solubilizing capacity of Bacillus cereus CLY07 was better in the modified NBRIP culture medium than in the Mengjinna organic culture medium. The phosphate solubilizing capacity of Bacillus cereus CLY07 reached the peak when the culture was supplied with glucose as carbon source, ammonium sulfate as nitrogen source and 60/1 as the carbon nitrogen ratio. The optimal culture condition was the pH value of 70, the temperature of 30℃, without NaCl and 20mL fluid culture in 100mL conical flask. The study showed that compared with Mengjinna organic culture medium, the modified NBRIP culture medium was more suitable for the research of the phosphate solubilizing capacity of Bacillus cereus CLY07. In addition, cultivation environment affected the phosphate solubilizing capacity of Bacillus cereus CLY07.