含羞草素对肝癌细胞QGY-7703周期同步化的作用

Cyecle Synchronization of Human Hepatoma Cell QGY-7703 Induced by L-mimosine

恶性肿瘤是严重威胁人类健康的疾病之一。肿瘤的发生发展本质上就是细胞无限制的周期性生长、增殖和分化的失调。细胞同步化是研究肿瘤发生发展及治疗的基础。实验以人肝癌QGY‐7703细胞株为研究对象，考察含羞草素阻断对周期同步化的作用。并与胸腺嘧啶脱氧核苷阻断法相对照，研究含羞草素阻断去除后对QGY‐7703细胞周期的影响。结果显示：QGY‐7703细胞在含羞草素阻断后释放的第10 h进入S期，第20 h进入G2／M期，且最高同步率分别为G1期94．6％，S期86．0％。含羞草素能较好地使肝癌细胞株QGY‐7703同步化于G1期和S期。

Cancer is one of diseases w hich severely threaten human health .In essence ,tumorigenesis and tumor progression result from a disorder of unlimited periodic grow th ,proliferation and differentiation of cells .Cell synchronization is the foundation of researches on tumorigenesis ,tumor progression and treatment .Here ,we investigated the effect of L‐mimosine blocking on cycle synchronization by taking human .hepatoma cell QGY‐7703 . Furthermore , compared with thymidine blocking , the effect of L‐mimosine blocking removal on cell cycle of QGY‐7703 cells was investigated .The results show that QGY‐7703 cells enter Sphase at the 10th hour and enter G2/M phase at the 20th hour after release from the L‐mimosine .The highest synchronization rate is 94 .6% in G1 phase and 86 .0% in S phase cells . In conclusion ,QGY‐7703 cell can be successfully synchronized in G1 phase and S phase by the L‐mimosine treatment .