总丹参酮中丹参酮ⅡA的提取分离

Extraction and purification of tashinon from salvia miltiorrhiza Bge

目的:以丹参酮ⅡA含量为跟踪指标,建立一个适合工厂化生产的丹参酮ⅡA分离纯化工艺。方法:先对提取溶剂、提取方法、提取溶剂用量和提取时间进行比较筛选,再利用硅胶柱层析进行分离纯化。结果:先用60倍的甲醇超声提取2.5h,再进过硅胶柱层析,以氯仿:石油醚(4∶1)为洗脱剂的提取纯化方法为最佳的工厂化生产工艺。结论:该工艺不仅提取率高,收率较高、成本低、合理,而且简便、实用。

Take the salvia miltiorrhiza alkone ⅡA content as the track target, a separation purification craft which is suitable to the factorization production of the Tanshinone ⅡA was established. The withdraws solvent,method, solvent amount and time was compared and selected, then using the silicagel column chromatographic analysis to carry on the separation purification. 60 times methyl alcohol supersonic extracted 2.5h first, then en-tered the silicagel column chromatographic analysis, by chloroform-Benzine（3∶1） for elutes the medicinal preparation to withdraw the purification method for the best factorization production craft. This craft has high extraction rate, and high yield, low cost, Moreover simple and practical