消瘤藤醇提物对H_(22)荷瘤鼠的抑瘤作用研究

Antitumor Effect of Alcohol Extract of Pileostegia tomentella on H_(22) Tumor-Bearing Mice

目的探讨消瘤藤乙醇提取物(PTAE)对H_(22)荷瘤小鼠肿瘤的抑制作用及其可能机制。方法选取小鼠60只,留10只作为正常对照组,其余小鼠均接种H_(22)荷瘤造模,造模成功后小鼠随机分为5组:模型对照组,氟尿嘧啶组(20 mg·kg^(-1)),消瘤藤乙醇提取物高、中、低剂量组(生药180、90、45 g·kg^(-1)),每天给药1次,连续给药10 d后各组小鼠眼球取血,分离血清,酶联免疫吸附法(ELISA)测定血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)含量;剥离瘤块、脾脏和胸腺,称重并计算抑瘤率、脾指数和胸腺指数;HE染色观察肿瘤组织病理学改变。结果与模型对照组比较,消瘤藤乙醇提取物高、中剂量组瘤重均明显降低(P<0.01,P<0.05),抑瘤率分别为37.44%和38.46%;消瘤藤乙醇提取物中剂量可显著提高荷瘤鼠脾脏指数(P<0.01);消瘤藤乙醇提取物高剂量组能显著升高血清白细胞介素-2水平(P<0.05);消瘤藤乙醇提取物高、中、低剂量组还可显著升高血清肿瘤坏死因子-α水平(P<0.01)。结论消瘤藤乙醇提取物具有抗肿瘤作用,其机制可能与调节机体细胞免疫功能有关。

OBJECTIVE To study the antitumor effect of Pileostegia tomentella 95% alcohol extract（PTAE） on H_（22）tumor-bearing mice and its possible mechanisms.METHODS Sixty mice were chosen and mouse models bearing H22 solid tumor were established in fifty mice,and the others were as normal control.H_（22）tumor-bearing mice were randomly divided into five groups：model control group,fluorouracil group（20 mg·kg^（-1））,PTAE high,middle and low-dose group（180,90,45 g·kg^（-1）of crude drug,respectively）.The mice in treatment groups were intragastric administration respectively,meanwhile,the mice in normal control and model groups were treated with the same volume of distilled water,once a day for ten days.The blood was collected from eyeball in all mice,and the serum were separated and detected by ELISA for IL-2 and TNF-α.Then the mice were put to death.Their tumors,thymuses and spleens were separated and weighted,and the tumor inhibitory rates,thymus and spleen indexes were calculated.The pathological change of tumor tissue was observed.RESULTS Compared with model control group,the tumor weights of PTAE high and middledose groups were significantly decreased（P〈0.05,P〈0.01）,the tumor inhibitory rates were 37.44% and 38.46% respectively.The spleen index of PTAE middle-dose group was increased significantly（P〈0.01）.The level of IL-2 in serum of tumor-bearing mice in the PTAE high-dose group was increased significantly（P〈0.05）,and the level of TNF-α in serum（P〈0.01） could be increased significantly in the PATE high,middle and low-dose groups.CONCLUSION Pileostegia tomentella 95% alcohol extract has antitumor activity,its mechanism may be developed by immuno-regulation.