心肌层表达tRNA源性小片段RNA

Expression of tRNA-derived fragments in myocardium

目的 研究大鼠左心室前壁（相当于心肌发生梗死的梗死区和边缘区）是否存在转移核糖核酸来源的小片段RNA（tRFs）的表达。方法 分离出大鼠左心室前壁（相当于心肌发生梗死的梗死区和边缘区）的心肌组织并提取总RNA。使用Illumina技术对小片段RNA进行测序,以获的该两个区域的50 nt以下小片段RNA的序列及表达量信息。利用Fstax和Bowtie软件,及Perl语言编程对长度为30-36 nt部分的小片段RNA进行注释,并比较两个区域tRF表达量差异。结果 大鼠心脏左心室前壁（相当于心肌发生梗死的梗死区和边缘区）的心肌组织存在tRF表达,而来源于tRNA的tRFs分别占总小片段RNA的9.15%和7.30%。且两个区域的经鉴别的28中tRFs种类及表达量十分接近。无论是相当于梗死区还是相当于边缘区的心肌组织,Gly GCC和Val CAC两个tRNA来源的tRFs占了所有tRFs的84.73%和84.66%。每一种tRNA来源的tRFs在两个区域间的表达差异小于4%。结论 生理状态下心肌组织存在tRF表达,相当于心肌发生梗死的梗死区和边缘区的心肌tRFs表达谱是十分接近的。

Objective To study whether the transfer RNA （tRNA） derived fragments （tRFs） is expressed in myo- cardium. Methods The anterior wall of left ventricle tissues which are matched to infarct zone and border zone were isolated and extracted the total RNA. The small RNA sequencing was conducted by the illumina sequencer. The sequences and dosage of expression were acquired. The software of Fastax and Bowtie, and perl language pro- gram were applied to annotate small RNAs with length between 30 and 36 nt and compare the difference between the two area cardiac tissues. Results The tRFs were found to be expressed in the anterior wall of left ventricle tis- sues which were matched to infarct zone and border zone. In addition, it was found that the percentages of tRFs inmalehed to infarct zone and border zone were 9. 15% and 7.30% comparing with total small RNA. The identified 28 tRFs and their expression were similar between the two areas. The percentages of two tRFs, GlyGC and Val- CAC, were 84.73% 和84.66% between the two areas. The expression difference between each tRFs in two areas was less than 4%. Conclusions tRFs are expressed in myocardium. The expression profile of tRFs in the myocar- dial lissues which are matched to infarct zone and border zone in myocardium is similar.