MYH9在人肝癌组织中的表达及其对肝细胞系增殖和凋亡的影响

Expression of MYH9 in human hepatocellular carcinoma tissue and its effects on proliferation and apoptosis of hepatocellular carcinoma cell line

目的探讨非肌肉肌球蛋白重链(MYH9)在肝癌组织中的表达及通过沉默MYH9基因对肝癌细胞系SMMC-7721的增殖及凋亡的影响。方法收集50组人肝癌组织及癌旁组织,选用人肝癌细胞系SMMC-7721和Hep G2及人正常肝细胞系LO2,免疫组织化学方法及Western blot检测肝癌组织及癌旁组织中MYH9蛋白的表达,Western blot检测SMMC-7721、Hep G2及LO2中MYH9蛋白的表达;将MYH9 siRNA转染SMMC-7721,CKK8法及流式细胞术检测沉默MYH9对肝癌细胞增殖及细胞凋亡的影响。结果 MYH9蛋白在肝癌组织中的表达明显高于癌旁(P<0.05);MYH9蛋白在SMMC-7721及Hep G2中的表达均明显高于LO2(P<0.05);沉默MYH9基因可抑制细胞增殖(P<0.001),促进细胞凋亡(P<0.05)。结论 MYH9蛋白在肝癌组织的表达显著高于癌旁组织;MYH9低表达能有效抑制肝癌细胞的增殖,促进其凋亡。

Objective To analyze the expression of nonmuscle myosin heavy chain9 （MYH9） in the human hepato- cellular carcinoma（HCC） and to investigate the effects of silencing MYtD gene on the proliferation and apoptosis of HCC cell line SMMC-7721. Methods Immunohistochemistry and Western blot were used to analyze the expression of MYH9 protein in 50 cases of HCC tissues and paraneoplastic tissues in HCC tissues and cancer-adjacent tissues, and the relationship between MYH9 and clinical pathological parameters was studied. Western blot was also used to ana- lyze the expression of MYH9 protein in HCC cell lines SMMC-7721, HepG2 and normal liver cell LO2. RNA interfer- ence targeted silence MYH9 gene was transfeeted into SMMC-7721 cells. The proliferation and apoptosis of the trans- letted cells were assessed by CCK-8 assay and flow cytometry. Results The expression of MYH9 in HCC tissues was found to be significantly higher than that of cancer-adjacent tissues（ P 〈 0. 05 ）, and the relative amount of MYH9 pro- tein in SMMC-7721 and HepG2 was significantly higher than that in LO2（P 〈 0. 05 ）. The downregulation of MYH9 inhibited cell proliferation（P 〈0. 05） and induced the apoptosis（P 〈0. 05）. Conclusions The expression of MYH9 protein in HCC tissues is significantly higher than that in cancer-adjacent tissues, and RNAi of MYH9 in the SMMC- 7721 cells may inhibit cells proliferation and induce apoptosis.