TRIM69可抑制紫外线和依托泊苷刺激时HeLa和HEK293T细胞系内活化型caspase7和剪切型PARP的表达

TRIM69 inhibits the expression of cleaved caspase7 and PARP in He La and HEK293T cell line with the treatment of UV and etoposide

目的验证TRIM69蛋白是否能够抑制He La和HEK293T细胞系内活化型caspase7(cleaved caspase7)和剪切型PARP(cleaved PARP)的蛋白水平。方法构建含人源TRIM69的真核表达质粒,转染He La细胞和HEK293T细胞,筛选稳定表达TRIM69的细胞系,通过紫外线照射和凋亡诱导剂依托泊苷(etoposide)刺激处理,Western blot检测细胞内TRIM69蛋白、活化型caspase7和剪切型PARP蛋白水平的变化。结果紫外线照射和依托泊苷刺激后,与对照组相比,TRIM69过表达的细胞内活化型caspase7和剪切型PARP的蛋白水平均下降(P<0.05)。结论TRIM69蛋白的表达可抑制紫外线或依托泊苷刺激时He La和HEK293T细胞内凋亡相关蛋白活化型caspase7和剪切型PARP的蛋白水平。

Objective To identify whether human TRIM69 may inhibit the expression of cleaved capase7 and PARP in HeLa and HEK293T cell line in response to UV irradiation or stimulation. Methods The gene of human TRIM69 was cloned into eukaryotic expression vectors, which was then transfected into HeLa or HEK293T cells. The cells were exposed to UV irradiation or stimulated with etoposide and then collected for western blot to detected the level of activation of caspase7 and the cleavage of PARP. Results The levels of expression of cleaved caspase7 and PARP showed a significant decline in the stable cells with ectopically ex- pressed TRIM69 comparing with the control one （P 〈 0.05 ）. Conclusions Expressed TRIM69 inhibites the level of expression of cleaved caspase7 and PARP in HeLa and HEK293T cells in response to both UV irradia- tion and etoposide stimulation.