罗丹明B酶联免疫分析方法的建立与应用

Establishment and application of enzyme-linked immuno sorbent assay(ELISA) for Rhodamine B

建立一种用于检测罗丹明B的间接竞争酶联免疫分析方法。该方法的IC50为1.3ng/m L,检测限为0.786ng/g。该方法检测豆瓣酱,加标浓度5 ng/g,平均回收率为83%,加标浓度10 ng/g,平均回收率为89%;检测辣椒酱,加标浓度5 ng/g,均回收率为88%,加标浓度10 ng/g,平均回收率为104%;检测辣椒油,加标浓度5 ng/g,平均回收率为94%,加标浓度10 ng/g,平均回收率为101%;检测辣椒粉,添加浓度5 ng/g,平均回收率为82%,加标浓度10 ng/g,平均回收率为90%。该方法的平均批内差为9.0%,批间差为10.5%,与其他常见染料未见交叉反应。该方法操作简便,结果准确,适用于罗丹明B的现场大量样品快速检测。

A fast assay method of enzyme-linked immuno sorbent assay（ELISA） for Rhodamine B（Rh B） is established. The method is characterized into that IC50 is 1.3 ng/m L and the limit of detection is 0.786 ng/g. For the detected bean sauce by the method,its average recovery rate is83% at the fortified concentration of 5 ng/g and 89% at the fortified concentration of 10 ng/g. For the detected chili sauce,its average recovery rate is 88% at the fortified concentration of 5 ng/g and 104% at the fortified concentration of 10 ng/g. For the detected chili oil,its average recovery rate is 94% at the fortified concentration of 5 ng/g and 101% at the fortified concentration of10 ng/g. For the detected chili powder, its average recovery rate is 82% at the fortified concentration of 5 ng/g and 90% at the fortified concentration of 10 ng/g. The intra assay CV of the ELISA method is 9.0%, and the inter assay CV is 10.5%. The ELISA method has no cross reaction with other common dyes. The method is featured with simple and convenient operation and accurate result, and it is applicable to rapid detection of a great number of Rhodamine B samples on site.