微生物检验标本分析前质量控制

Pre-examination Quality Control of Microbiological Testing Specimens

目的探讨分析微生物检验标本不合格的原因,探讨微生物检验质量控制的临床操作规范。方法回顾2014年以来该院检验科收到的121份不合格微生物检验标本,计算各类不合格标本的构成比,分析不合格标本出现的主要原因。结果 121份不合格标本中,痰液标本55份,占45.45%;尿液标本25份,占20.66%;血液标本19份,占15.70%;分泌物标本12份,占9.92%;粪便标本7份,占5.79%;无菌体液标本3份,占2.48%。标本污染、采集时间错误和送检不及时为不合格标本的主要原因。结论正确、规范的采集、留取、储存、运输和处理标本是微生物检验标本分析前质量控制的前提,关键在于检验人员应与医护人员密切配合,切实做到规范操作。

Objective To explore the reasons for the failure of microbiological test specimens, development of clinical practices microbiological testing quality control. Methods Recalling since 2014 in our hospital laboratory received 121 samples failed microbiological testing to calculate all kinds of unqualified specimen constituent ratio, mainly appear unqualified specimen analysis. Results 121 failed samples, sputum samples 55, accounting for 45.45%; 25 urine samples, accounting for 20.66%; 19 blood samples, accounting for 15.70%; secretion samples 12, accounting for 9.92%; seven stool specimens,accounting for 5.79%; sterile body fluid samples 3, accounting for 2.48%. Specimen contamination, acquisition time errors and untimely submission are the main reasons for the failure of the specimen. Conclusion Correct, standardized collection,specimens, storage, transport and processing specimen samples are premise on pre-examination quality control of microbiological testing specimens, the key is that inspectors should work closely with the medical staff, and effectively regulate the operation.