羊膜提取液治疗兔干眼症的实验研究

Therapeutic efficiency of amniotic extraction for dry eye rabbit model

目的:研究羊膜提取液对苯扎氯铵诱导的兔干眼的治疗作用。方法:选择新西兰大白兔26只(26眼,均为右眼),使用0.2%的苯扎氯铵制作兔干眼症模型,随机分为两组:对照组(A组,13眼)使用磷酸盐缓冲液(phosphate buffered saline,PBS)滴眼,实验组(B组,13眼)使用1%羊膜提取液(amniotic extraction)滴眼,4次/d。另选取2只兔作为正常对照。分别于治疗前和治疗1、2、4、8wk行Schirmer I试验(SchirmerⅠtest,SⅠt)、角膜荧光素染色(corneal fluorescein staining,FL)、泪液总蛋白含量、淀粉酶活性、乳铁蛋白、溶菌酶含量检测,并进行结膜印迹细胞检查。结果:不同组间、不同时间点间SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异有统计学意义(P<0.05)。治疗前两组SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异无统计学意义(P>0.05);治疗1、2、4、8wk后,B组SⅠt、FL、溶菌酶活性较治疗前均有不同程度改善,差异有统计学意义(P<0.05),B组结膜杯状细胞密度与治疗前相比有所增加,但差异无统计学意义(P>0.05);治疗1、2、4、8wk后,A组SⅠt、溶菌酶活性及结膜杯状细胞密度均有不同程度下降,差异有统计学意义(P<0.05),A组FL评分与治疗前相比有不同程度增加,但差异均无统计学意义(P>0.05)。A、B两组各时间点SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异均有统计学意义(P<0.05)。同一组内不同时间点,泪液中总蛋白量、乳铁蛋白、淀粉酶活性差异无统计学意义(P>0.05)。治疗前A、B两组泪液中总蛋白量、乳铁蛋白、淀粉酶的活性相比,差异无统计学意义(P>0.05);治疗4、8wk后两组泪液中总蛋白量、乳铁蛋白、淀粉酶的活性相比,其差异有统计学意义(P<0.05)。结论:羊膜提取液能有效治疗兔干眼症,具有良好的临床应用前景。

AIM： To investigate therapeutic efficiency of amniotic extraction on dry eye in rabbit model induced by topical benzalkonium chloride （BAC）. METHODS： Totally 26 rabbits （26 right eyes） with dry eye model were studied and divided into two groups：group A （control group with PBS eye drops, n = 13） and group B （ amniotic extraction group, n = 13）. Another two rabbits were chosen as normal control. The SchirmerⅠ tests （ S Ⅰ t） and corneal fluorescein staining （ FL） were made, and the tear total protein content, amylase activity, lactoferrin, lysozyme contents, goblet cell density were performed in two groups before treatment and 1, 2, 4 and 8 wk after treatment. RESULTS： There were significant differences in SIT, FL scores, lysozyme activity and goblet cell density among different groups at different time points （P〈0. 05）. But,＆amp;nbsp;there was no significant differences in SⅠt, FL scores, lysozyme activity and goblet cell density between two groups before treatment （P〉0. 05）. After 8wks＆#39; treatment with PBS, the mean differences of the group A showed great changes in SⅠt, lysozyme and goblet cell density compared with those before treatment （ P 〈 0. 05）; but there was no significant differences in FL scores compared with those before treatment （P〉0. 05）. As for group B, 8wks after treatment, there were statistical changes in SⅠt, FL, lysozyme （P〈0. 05）; but there was no significant differences in goblet cell density compared with those before treatment （ P 〉 0. 05）. It was evident that statistical differences were observed in S Ⅰ t, FL scores, lysozyme activity and goblet cell density between two groups at each time point （P〈0. 05）. However, there were no significant differences in total protein, lactoferrin, amylase activity at different time points （P〉0. 05）. Meanwhile there was no significant differences in total protein, lactoferrin, amylase activity between two groups before treatment （ P 〉 0. 05 ）. But there were significant differences in total protein, lactoferrin, amylase activity between two groups after 4 and 8 wks＆#39;treatment （P〈0. 05）. CONCLUSION： Amniotic extraction has significant therapeutic effect on the dry eye in rabbit model.