银杏内酯B对高糖诱导人晶状体上皮细胞凋亡的影响

Effect of ginkgolide B on high glucose-induced human lens epithelial cell apoptosis

目的:探讨银杏内酯B对高糖诱导的人晶状体上皮细胞(human lens epithelial cells,HLECs)凋亡的影响。方法:建立高糖诱导的HLECs模型,用不同浓度的银杏内酯B干预,MTT比色法检测细胞存活率,Hochest33258染色观察凋亡细胞形态,透射电镜观察细胞内超微结构变化,比色法检测凋亡相关因子Caspase-3及Caspase-9的表达。结果:高浓度葡萄糖可抑制HLECs活性,诱导HLECs发生凋亡,引起细胞内Caspase-3及Caspase-9表达水平升高;银杏内酯B能明显抑制高糖诱导的HLECs活力的下降,降低细胞凋亡率,减少高糖所致HLECs细胞内Caspase-3及Caspase-9的表达。结论:银杏内酯B可能通过抑制Caspase-3及Caspase-9的表达而有效抑制了高糖诱导的HLECs凋亡。

AIM： To study the effect of ginkgolide B on high glucose-induced human lens epithelial cell （HLEC） apoptosis. METHODS： The high glucose-induced HLEC model was established. Different concentrations of ginkgolide B were intervened. Cell viability was assayed by MTT assay, morphology of cell apoptosis was observed by hochest33258 staining. Cell ultrastructural changes were detected by transmission electron microscopy. Expressions of apoptosis - related factors caspses - 3 and caspase-9 were tested by colorimetric detection. RESULTS： High glucose inhibited the activity of HLECs, induced apoptosis reaction of HLECs, caused high expression of apoptosis factors in cells; while ginkgolide B inhibited the decrease of cell viability induced by high glucose, decreased the HLEC apoptosis and reduced the expression of apoptosis factors Caspase-3 and Caspase-9.CONCLUSION： Ginkgolide B may inhibit the expression of caspses-3 and caspase-9 then effectively inhibited high glucose-induced apoptosis in HLECs.