头花蓼对淋巴细胞分泌干扰素γ和白介素4的影响

Influence of Polygonum capitatum on IFN-γand IL-4 Secretion in Spleen Lymphocytes

目的：研究苗药头花蓼对淋巴细胞分泌干扰素1（IFN-γ）及白介素4（IL-4）的影响。方法：制备C57BL／6小鼠脾淋巴细胞悬液，同刀豆蛋白A（ConA）及不同浓度的头花蓼（终浓度分别为：1、2、4、8、16、32、64、128、256、512mg／L）于37℃、5％CO2培养箱共培养72h，细胞活性检测试剂盒（CCK-8）检测头花蓼对体外T淋巴细胞增殖的影响，酶联免疫吸附实验（ELISA）测定细胞上清中的IFN-γ和IL-4含量，分析不同浓度的头花蓼对ConA活化的脾淋巴细胞增殖和分泌IFN-γ、IL-4的影响。结果：头花蓼浓度〉128mg／L时，明显抑制T淋巴细胞的增殖，抑制其分泌IFN-γ的水平，与空白组比较差异有统计学意义（P〈0．05）；头花蓼浓度为8～128mg／L时，ConA活化的脾淋巴细胞分泌IFN-γ和IL-4的水平明显高于空白对照，而T淋巴细胞的增殖无明显变化；头花蓼的浓度低于32mg／L时，ConA活化的淋巴细胞的IFN-γ分泌水平随头花蓼浓度升高而增加。结论：一定浓度的头花蓼刺激小鼠脾淋巴细胞增殖、IFN-γ和IL-4分泌，参与机体细胞免疫过程。

Objective ： To investigate the effect of Polygonum capitatum on the secretion of IFN-γ and IL-4 in mice spleen lymphocytes. Methods： A series of concentrations of Polygonum capitatum （final concentrations were 1,2,4,8,16,32,64,128,256,512 mg/L） in splenic lymphocytes of C57BL/6 mice that were activated by ConA for 72 h at the condition of 37 ℃ and 5% CO2. Following Counting Kit-8 was used to detected lymphocytes proliferation and double antibody sandiwich ELISA was used to detect changes, the effect of Polygonum capitatum with different concentration on proliferation and se- cretion of IFN-γ and IL-4 was analyzed. Results： The T-cell proliferation and IFN-γsecretion was re- strained when the concentration of Polygonum capitatum was higher than 128 mg/L. The level of IFN- γand IL-4 secreted by mice spleen lymphocytes after ConA activation was higher obviously with Polyg- onum capitatum at the concentration between 8 to128 mg/L, compared with the blank control group （P 〈 0.05 ）. T-cell proliferation had no obvious change. When the concentration of Polygonum capitatum was lower than 32 mg/L, secretion amount of IFN-γ increased as the concentration of Polygonum capi- tatum become higher. Conclusion： Polygonum capitatum at a certain concentration could participatein cellular immunity that stimulate the mice spleen lymphocytes proliferation, and secretion IFN-γ and IL-4 in vitro .