中性粒细胞碱性磷酸酶染色阳性对照品设计

A Preliminary Study on the Design of Positive Control of Neutrophils Alkaline Phosphatase Staining

目的:探讨EDTA-K_2抗凝外周血作为中性粒细胞碱性磷酸酶(NAP)染色阳性对照的可能性。方法:选取EDTA抗凝的NAP积分正常外周静脉血及NAP积分明显升高外周血,各制成200张血片,分为积分正常组和积分升高组,这两组又再分为不固定组和固定组(用10%甲醛—甲醇固定);每隔5 d每组取10张血片进行NAP染色,共计50 d染色10次,比较各组NAP阳性率及积分。结果:每组血片中NAP活性随着时间推移而减弱,积分明显升高的外周血NAP活性能维持到40 d,10%甲醛-甲醇固定的血片酶活性下降相对缓慢。结论:选用NAP积分明显升高的外周血,经10%甲醛-甲醇固定后可作为NAP染色的阳性对照,其有效期约为40 d。

Objective： To explore the possibility of EDTA-K2 anticoagulant peripheral blood as stai- ning positive control of neutrophil alkaline phosphatase （NAP）. Methods ： Peripheral venous blood of EDTA anticoagulation with normal NAP score and peripheral venous blood of EDTA anticoagulation with significantly increased NAP integral were selected to make 200 blood pieces, respectively. They were divided into normal NAP score group and increased NAP integral group, and they were further di- vided into non fixed group and fixed group （fixed by 10% formaldehyde-methanol）. Nap staining was performed every other week at 5 d in each of the 10 groups, with a total of 50 d staining for 10 times. NAP positive rates and score were compared between each group. Results： The activity of NAP de- creased with time in blood pieces of each group. Peripheral venous blood with significantly increased NAP integral can be maintained for about 40 days. The activity of enzyme in the blood fixed by 10% formaldehyde-methanol decreased relatively slowly. Conclusion： Peripheral venous blood with signifi- cantly increased NAP integral after fixed by 10% formaldehyde-methanol can be selected to be staining positive control of NAP, and the effective period is about 40 days.