银杏叶原花青素的制备及其抗氧化活性研究

Proanthocyanidins from Ginkgo Biloba L. and Their Anti-oxidant Activities

目的制备高纯度的银杏原花青素,为系统研究银杏来源的原花青素提供物质基础。方法利用NP-HPLC方法指导大孔树脂及聚酰胺树脂的纯化工艺,分析并定量所得原花青素提取物中黄酮杂质的组成及含量,用DPPH法比较银杏和葡萄籽来源的原花青素的抗氧化活性。结果制备得到的银杏原花青素提取物中原花青素的含量>90%,黄酮杂质含量仅为3.44%,DPPH清除率IC50为22.87μg·mL^(-1),葡萄籽原花青素IC50为36.99μg·mL^(-1)。结论本研究工艺可制备获得高纯度的银杏原花青素,其抗氧化活性较葡萄籽原花青素更强。

OBJECTIVE To prepare highly purified proanthocyanidins from Ginkgo biloba L., so as to provide material base for the systematic study of proanthocyanidins from Ginkgo biloba L.. METHODS The purification processes of macroporous resins and polyamide resin were optimized by using NP-HPLC methods; qualitative and quantitative analysis of flavonoid, the impurity of Ginkgo biloba proanthocyanidin extract（GPE）, was realized by HPLC with different chromatographic conditions; the anti-oxidant activities of proanthocyanidins from Ginkgo biloba L. and grape seed were compared by DPPH assay. RESULTS The purity of proanthocyanidins in GPE was 〉90%, and the content of flavonoid was only 3.44%. The GPE exhibited higher DPPH radical scavenging activity with IC50 22.87 μg·mL^-1 than that of grape-seed proanthocyanidins with IC5036.99 μg·mL^-1. CONCLUSION The process of this study could be prepared to obtain high-purity GPE, and its antioxidant activity is better than grape-seed proanthocyanidin.