期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

基于prrnS基因序列分析黑斑蛙裂头蚴种系发育关系

Phylogenetic Analysis of Mitochondrial PrrnS Genes of Frog Sparganum
下载PDF
导出
摘要 为研究湖南省黑斑蛙裂头蚴分离株的核糖体(rDNA)小亚基基因(rrnS)部分序列(prrnS)的遗传变异情况,并用所获得prrnS序列构建黑斑蛙裂头蚴与其他绦虫的种群遗传关系。利用聚合酶链反应(PCR)扩增湖南省黑斑蛙裂头蚴的prrnS,将PCR产物进行测序并对其序列进行分析。9株湖南省黑斑蛙裂头蚴分离株的prrnS序列长度均一致,为330 bp。经分析显示,黑斑蛙裂头蚴prrnS序列种内相对保守,种间差异较大,故可作为蛙裂头蚴的种间遗传变异研究的分子遗传标记。 The purposes of the present experiment are to examine sequence variation in the small subunit ribosomal RNA （rrnS） gene of F.sparganum isolates from Hunan province, and to construct their phylogenetie relationship based on rrnS sequences. The partial rrnS （prrnS） was amplified from each F. sparganum sample by PCR and PCR product was sequenced. The results showed that the lengths of prrnS sequence were 330 bp. The results indicated that there was no significant variation in rrnS sequences within F.sparganum, while intra-species difference was obvious. It was concluded that prrnS sequences could be used as genetic marker for population genetic studies of eestodes.
作者 侯强红 谭磊 邓兴珍 刘伟
机构地区 怀化职业技术学院动物科技系 湖南农业大学动物医学院 沅陵县畜牧水产局
出处 《经济动物学报》 CAS 2016年第2期74-77,共4页 Journal of Economic Animal
基金 湖南省科技计划一般项目(2014NK3049) 湖南省畜牧局资助项目
关键词 黑斑蛙 裂头蚴 核糖体DNA prrnS基因 序列分析 种系发育关系 frog sparganum ribosome DNA rrnS gene sequence analysis relationship
分类号 S852.734 [农业科学—基础兽医学]
  • 相关文献

参考文献10

  • 1Nachamkin I,Allos B M,Ho T.Campylobacter species and Guillain-Barrésyndrome[J].Clin Microbiol Rev,1998,11(3):555-567.
  • 2Wassenaar T M,Fry B N,Lastovica A J,et al.Genetic characterization of Campylobacter jejuni O:41 isolates in relation with Guillain-Barrésyndrome[J].J Clin Microbiol,2000,38(2):874-876.
  • 3Koga M,Gilbert M,Takahashi M,et al.Comprehensive analysis of bacterial risk factors for the development of Guillain-Barrésyndromeafter Campylobacter jejuni enteritis[J].J Infect Dis,2006,193(4):547-555.
  • 4van Den Braak N,Duim B,Rigter A,et al.Molecular characterization of Campylobacter jejuni from patients with GuillainBarréand Miller Fisher syndromes[J].J Clin Microbiol,2000,38(6):2297-2301.
  • 5Endtz H P,Ang C W,Mc Manus D P,et al.Molecular genetic approaches to parasite identification:their value in diagnostic parasitology and systematics[J].Int Paras-itol,1996,26(7):687-704.
  • 6Nadler S A.Molecular approaches to studying helminth population genetics and phylogeny[J].Int J Parasitol,1990,20(1):11-29.
  • 7Pirchard R.Application of molecular biology in veterinary Parasitology[J].Vet Parasitol,1997(71):155-175.
  • 8Chilton N B,Gasser R B,Beveridge I.Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex(Nematoda:Strongyloidea)[J].International Journal for Parasitology,1995,25(5):647-651.
  • 9Liu G H,Lin R Q,Li M W,et al.The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans[J].Molecular Biological Report,2001,38(4):2249-2256.
  • 10刘自逵,赵光辉,刘国华,宋海燕,李芬,刘毅.湖南省猬迭宫绦虫的线粒体nad5和rrnS基因的序列测定及种系发育分析[J].中国兽医学报,2012,32(2):258-261. 被引量：9

二级参考文献18

  • 1Murata K, Abe T, Gohda M,et al. Difficulty in diagnosing a case with apparent sequel cerebral sparganosi [J]. Surg Neurol,2007,67(4) :409-412.
  • 2lwatani K, Kubota I, Hirotsu Y,et al. Sparganum mansoni parasitic infection in the lung showing a nodule [J]. Pathol Int,2006,56(11) :674-677.
  • 3Nobayashi M, Hirabayashi H, Sakaki T,et al. Surgical reruov al of a live worm by stereotactic targeting in cerebral sparga nosis. Case report [J]. Neurol Med Chit, 2006,46 (3) : 164167.
  • 4Li M W, Lin H Y, Xie W T, et al. Enzootic Sparganosis in Guangdong,People's Republic of China[J]. Emerg Infect Dis, 2009,15 : 1317-1318.
  • 5McManus D P,Bowles J. Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics [J]. Int J Parasitol,1996,26(7) :687-704.
  • 6Gasser R B,Newton S E. Genomic and genetic research on bur sate nematodes: significance, implications and prospects [J]. Int J Parasitol,2000,30(4):509-534.
  • 7Blouin M S. Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer [J]. Int J Par asitol, 2002,32 (5) : 527-531.
  • 8Hu M,Chilton N B,Gasser R B. The mitochodrial genomies of para sitic nematodes of importance: recent progress, and implications for population genetics and systematies [J]. Adv Parasitoi,2004,56:133-212.
  • 9Boore J L. Animal mitochondrial genomes[J]. Nucleic Acids Res, 1999,27(8) : 1767-1780.
  • 10Hu M,Gasser R B,Abs E L,et al. Structure and organization of the mitochondrial genome of Dirofilaria immitis[J]. Par asitology,2003,127(1) :37-51.

共引文献8

经济动物学报
2016年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部