桑皮提取物中1-脱氧野尻霉素的气液相色谱分析方法比较

The Comparison of Gas Chromatography and Liquid Chromatography Analysis Methods for 1-DNJ in Mulberry Extract

文章分别采用气相色谱法和液相色谱法对1-脱氧野尻霉素(1-DNJ)进行检测,并对这两种方法的最低检出限、回收率和相对标准偏差进行比较。结果表明,气相色谱法的最低检出限为60μg/g,低、中、高3个水平回收率分别为98.0%、99.9%、98.7%,相对标准偏差分别为3.10%、2.43%、2.63%;液相色谱法的最低检出限为15.0μg/g、低、中、高3个水平回收率分别为96.3%、95.6%、94.8%,相对标准偏差分别为5.35%、4.90%、4.74%。因此,对于1-脱氧野尻霉素(1-DNJ)的检测,气相色谱分析法优于液相色谱分析法。

In this work, 1-DNJ was characterized by gas chromatography and liquid chromatography, and the minimum detection limit, recovery rate, and relative standard deviation of these two methods were compared. The results showed that, for gas chromatography, the minimum detection limit was 60 μg/g, the value of low, middle and high level recovery rate was 98.0 %, 99.9 %, 98.7 % and the relative standard deviation was 3.10 %, 2.43%, 2.63%,respectively. For liquid chromatography, the minimum detection limit was 15.0 μg/g, the value of low, middle and high level recovery rate was 96.3 %, 95.6 %, 94.8 % and the relative standard deviation was 5.35 %, 4.90 %, 4.74 %, respectively. Therefore, for the detection of 1-DNJ, gas chromatography was superior to liquid chromatography.