不同方法提取小叶女贞果实挥发油的GC-MS分析

GC-MS Analysis of the Chemical Constituents of Essential Oil from Ligustrum quihoui Carr. Fruits by Different Extraction Methods

采用超临界CO2流体萃取法(SFE-CO2)和水蒸气蒸馏法(SD)提取小叶女贞果实中的挥发油成分,并运用气相色谱-质谱联用法(GC-MS)分离和分析这两种挥发油的化学成分,用峰面积归一化法确定各化合物的相对百分含量,比较两种不同提取方法所得挥发油组分的差异。结果表明两种方法提取的挥发油中,共鉴定了92种成分,其中共有成分10种。超临界CO2流体萃取法提取的挥发油中,共鉴定了54种成分,占挥发油总量的73.18%,含量最高的组分为羽扇豆醇(27.55%);水蒸气蒸馏法提取的挥发油中,共鉴定了48种成分,占挥发油总量的69.74%,含量最高的组分为13-epi-泪柏醚(13.04%)。两种方法提取小叶女贞果实挥发油的化学组分与含量差别较大,因此可根据生产需要,选用不同方法提取小叶女贞果实中的有效成分。

Essential oil from Ligustrum quihoui Carr. fruits is extracted by supercritical CO2 fluid extraction （SFE-CO2）and steam distillation （SD）. The constituents of the essential oil are separated and analyzed by gas chromatography-mass spectrometry （GC-MS）. The relative percentage of the constituents is calculated by area normalization method. The chemical constituents of the essential oil by two different extraction methods are compared. The results show that ninety-two compounds including ten common components are identified totally from the essential oil by the two different methods. Fifty-four and forty- eight constituents, which occupy 73.18% and 69.74% of the total essential oil constituents, are identified by SFE-CO2 and SD respectively. The components with the highest levels extracted by SFE-CO2 and SD are Lupeol （27.55%）and 13-epi-Manoyl oxide （13.04%） respectively. The constituents and content of the essential oil extracted by the two methods exhibit significant differences. Therefore, effective components from Ligustrum quihoui Carr. fruits can be extracted by different methods according to production needs.