银屑病患者损伤处皮肤间充质干细胞对T细胞增殖的影响

Influence of skin-derived mesenchymal stem cells from psoriasis lesions on T cell proliferation

背景:有研究发现银屑病患者骨髓间充质干细胞生物学活性异常,细胞因子分泌水平也有差异,但有关银屑病患者皮肤损伤处间充质干细胞的生物学活性研究较少。目的:探讨银屑病患者皮损间充质干细胞对T细胞增殖的影响。方法:分离和培养银屑病患者损伤皮肤间充质干细胞和正常皮肤间充质干细胞,分别与外周血T细胞共培养,MTT法检测外周血T细胞增殖能力。ELISA法检测皮肤间充质干细胞上清液中表皮细胞生长因子和转化生长因子β1水平。结果与结论:(1)银屑病患者皮肤间充质干细胞和正常皮肤间充质干细胞对T细胞增殖均有抑制作用,与T细胞自然增殖组比较差异均有显著性意义(P<0.05),银屑病患者皮肤间充质干细胞对T细胞增殖的抑制作用较弱;(2)银屑病患者皮肤间充质干细胞组上清液中表皮细胞生长因子水平高于正常皮肤间充质干细胞组(P<0.05),银屑病患者皮肤间充质干细胞组上清液中转化生长因子β1水平低于正常皮肤间充质干细胞组(P<0.05);(3)结果表明,银屑病患者皮肤间充质干细胞对T淋巴细胞的抑制作用减弱,可能为皮损处微环境中生长因子水平失衡所致。

BACKGROUND： Studies have found abnormalities in the biological activity and cytokine levels of bone marrow mesenchymal stem cells from psoriasis patients, while the biological activity of skin-derived mesenchymal stem cells from psoriasis lesions is rarely reported. OBJECTIVE： To explore the influences of skin-derived mesenchymal stem cells from psoriasis lesions on T cell proliferation METHODS： After isolation and culture, skin-derived mesenchymal stem cells from psoriasis lesions and normal controls were separately co-cultured with peripheral blood T cells. T cell proliferation was detected using MTT assay, and levels of epidermal growth factor and transforming growth factor β1 in cell supernatant determined using ELISA method. RESULTS AND CONCLUSION： Skin-derived mesenchymal stem cells from both psoriasis lesions and normal controls significantly inhibited T cell proliferation（P〈0.05）. Furthermore, skin-derived mesenchymal stem cells from psoriasis lesions exhibited a weaker inhibitory effect on T cell proliferation than normal skin-derived mesenchymal stem cells（P〈0.05）. Skin-derived mesenchymal stem cells from psoriasis lesions significantly increased epidermal growth factor but reduced transforming growth factor β1 compared with the normal skin-derived mesenchymal stem cells（P〈0.05）. In conclusion, maybe the imbalances of growth factor levels due to skin injury elicit a decrease in the inhibitory effect of skin-derived mesenchymal stem cells from psoriasis lesions on T lymphocytes.