干细胞、微环境与活血化瘀

Insight into stem cells, microenvironment and methods for promoting blood circulation and removing stasis

背景:干细胞、微环境与活血化瘀之间存在一定的相关性。目的:通过阐述干细胞、微环境与活血化瘀之间的联系,系统综述3者的相互影响,提出活血化瘀的"肾属性"观点。方法:以"bone mesenchymal stem cells,transformation,differentiation,blood-activation and stasis-resolved method,microenvironment"或"干细胞,微环境,活血化瘀"为主题检索词,检索中国期刊全文数据库、PubM ed、万方数据库中2009至2015年关于活血化瘀法促进干细胞增殖分化及与微环境相关的文章,选择其中的32篇进行综述。结果与结论:干细胞归巢到损伤组织将发生"环境依赖性分化",这种在受损区域形成的特殊环境对骨髓干细胞的分化和增殖起关键作用,在局部微环境诱导下骨髓干细胞分化成心肌细胞、血管内皮细胞等损伤组织细胞。微环境作为一个综合各指标动态、微观、发展的物质集合,在干细胞的生存、繁殖和分化诸多环节中起着至关重要作用。活血化瘀方药对干细胞"归巢"过程的多个环节、多个靶点均有调节作用,课题从理论层面探讨干细胞、微环境与活血化瘀相关性,并结合前期实验和临床研究所感,在活血化瘀法"祛瘀血"层面和"生新"层面理论研究基础上,进一步提取了活血化瘀的"肾属性"观点,指出干细胞具有肾中所藏精气的特质,是活血化瘀法生新执行的主体,是活血化瘀"肾属性"的物质基础,对完整揭示活血化瘀理论的科学内涵有一定价值。

BACKGROUND： There is a certain association between stem cells, microenvironment and methods for promoting blood circulation and removing stasis. OBJECTIVE： To propose a perspective of ＂kidney properties＂ of promoting blood circulation and removing stasis by elaborating the correlation and interactions of stem cells and microenvironment with methods for promoting blood circulation and removing stasis. METHODS： A computer-based search of Chinese Journal Full-text Database, Wanfang and Pub Med was performed for articles published form 2009 to 2015 addressing improving stem cell proliferation and differentiation by promoting blood circulation and removing stasis as well as microenvironments. The keywords were ＂bone mesenchymal stem cells, transformation, differentiation, blood-activation and stasis-resolved method, microenvironment＂ in Chinese and English, respectively. Finally, 32 literatures were reviewed. RESULTS AND CONCLUSION： Stem cells homing to the damaged tissue trigger an ＂environmentdependent differentiation＂, that is, stem cells can differentiate into myocardial cells, vascular endothelial cells and other injured tissues and cells under local special microenvironments. Microenvironment as an assembly for integrating dynamic, microscopic changes as well as development of a variety of indices is of great importance for stem cell survival, proliferation and differentiation. Recipes for promoting blood circulation and removing stasis are involved to regulate multi-steps and multi-targets during stem cell homing. Theoretically, this review attempts to investigate correlation among stem cells, microenvironment and methods for promoting blood circulation and removing stasis in association with previous preclinical and clinical findings. Here, we further refine the view of ＂kidney properties＂ of promoting blood circulation and removing stasis and clarify that stem cells as the material basis of ＂kidney properties＂ of promoting blood circulation and removing stasis is the main executor for promoting blood circulation and removing stasis. This paper is of a certain value for fully revealing the scientific connotation of the theory of promoting blood circulation and removing stasis.