针刺对脑性瘫痪模型大鼠神经细胞凋亡的影响

Effect of acupuncture on neural cell apoptosis of cerebral palsy rats

背景:研究表明针刺可以影响脑瘫动物的脑血流量、减轻大脑组织水肿、改善大脑微循环、减少神经细胞凋亡。目的:进一步验证针刺对脑瘫大鼠神经细胞凋亡的影响。方法:SD大鼠45只,随机分为3组:假手术组、模型组、针刺组,每组15只大鼠。模型组和针刺组大鼠结扎左侧颈总动脉建立脑瘫大鼠动物模型;假手术组找到左颈总动脉后不结扎。针刺组从造模后第2天开始进行针刺治疗,1次/d,连续针刺20 d,假手术组和模型组不予任何处理。手术后第21天观察大鼠治疗后的神经功能变化;取大鼠脑组织病理形态学改变,以及神经细胞的凋亡情况。结果与结论:(1)运动功能评分:模型组大鼠的均明显低于假手术组(P<0.05),针刺组在第21天时的运动功能评分明显高于模型组(P<0.05)。(2)苏木精-伊红染色结果:模型组大鼠大脑显示细胞排列紊乱,局部有炎症细胞浸润,见囊性变,神经细胞数量减少,并有变性坏死,胶质细胞增生,细胞核固缩,部分神经细胞有空腔形成,胞体缩小,结构模糊或者消失;假手术组大鼠大脑神经细胞形态和结构正常;针刺组大鼠大脑神经细胞的形态和结构改变介于假手术组和模型组之间。(3)细胞凋亡数目:模型组的神经细胞凋亡明显高于假手术组(P<0.05);针刺组的神经细胞凋亡数目也明显高于假手术组(P<0.05),但明显低于模型组(P<0.05)。(4)结果表明:针刺治疗可以改善脑性瘫痪大鼠的神经功能,减少脑性瘫痪大鼠的神经细胞凋亡,对脑性瘫痪大鼠的神经细胞有保护作用。

BACKGROUND： Acupuncture has been shown to impact cerebral blood flow, relieve cerebral edema, improve microcirculation and reduce neural cell apoptosis in animals with cerebral palsy. OBJECTIVE： To further verify the effect of acupuncture on neuronal apoptosis in cerebral palsy rats. METHODS： Forty-five Sprague-Dawley rats were randomly divided into sham group, model group and the acupuncture group, with 15 rats in each group. In the model and acupuncture groups, rat models of cerebral palsy were established by ligating the left common carotid artery. In the sham surgery group, the left common carotid artery was not ligated. Rats in the acupuncture group received acupuncture from 2 days after model induction, once a day, for 20 consecutive days. Rats in the sham surgery and model groups were left intact. At 21 days after surgery, neurological functions, pathomorphological changes and neuronal apoptosis were observed. RESULTS AND CONCLUSION：（1） Motor function score was significantly lower in the model group than in the sham surgery group（P〈0.05）. Motor function score was significantly higher in the acupuncture group than in the model group at 21 days（P〈0.05）.（2） Hematoxylin-eosin staining： In the model group, cells were scattered. Inflammatory cell infiltration and cystis degeneration were found. The number of nerve cells was reduced. Degeneration, necrosis, gliosis and karyopyknosis were detected. Cavitation was visible in some cells. Cell bodies became small. The structure was not distinct or disappeared. In the sham surgery group, the morphology and structure of nerve cells were normal. In the acupuncture group, the changes in morphology and structure of nerve cells were found between the sham surgery and model groups.（3） The number of apoptotic cells was significantly greater in the model group than in the sham surgery group（P〈0.05）. The number of apoptotic nerve cells was significantly higher in the acupuncture group than in the sham surgery group（P〈0.05）, but significantly lower than in the model group（P〈0.05）.（4） Results confirmed that acupuncture therapy can improve neurological function, reduce nerve cell apoptosis, and play a protective effect on nerve cells of rats with cerebral palsy.