长期有氧运动增加自发性高血压大鼠肾脏内源性硫化氢的生成

Long-term aerobic exercise enhances the production of endogenous hydrogen sulfide in the kidney of spontaneously hypertensive rats

背景:众所周知,长期有氧运动可以改善自发性高血压大鼠的肾脏功能,然而其中的机制尚未被完全阐明。目的:观察长期有氧运动对自发性高血压大鼠肾脏内源性硫化氢生成的影响。方法:构建长期有氧运动大鼠模型。实验随机分为4组,即WKY大鼠静止组、WKY大鼠运动组、自发性高血压大鼠静止组及自发性高血压大鼠运动组。运动为中等强度跑台运动,持续12周。造模完成后24 h称质量,尾动脉检测血压,收集血液和尿液检测肾脏功能相关的生化指标,取肾脏观察肾小球硬化程度,检测肾脏组织的硫化氢生成活性,RT-PCR法检测硫化氢生成相关酶的mRNA表达水平;同时考察肾脏组织的氧化应激情况。结果与结论:(1)经过长期的有氧运动之后,自发性高血压大鼠的体质量、收缩压、舒张压明显降低;肾小球滤过率和肾血流量增加,血肌酐和尿素氮水平降低,尿白蛋白水平降低,肾小球硬化评分明显降低;血浆中硫化氢含量和肾脏组织中硫化氢生成速率明显增加;胱硫醚γ分解酶表达明显上调;血清中和肾脏中丙二醛含量明显降低;还原型谷胱甘肽和氧化型谷胱甘肽比值明显增加;(2)结果提示,长期有氧运动可增加自发性高血压大鼠肾脏内源性硫化氢的生成,从而减轻肾脏组织的氧化应激,最终有助于维持自发性高血压大鼠的肾脏功能。

BACKGROUND： It is well known that long-term aerobic exercise alleviates renal dysfunction in spontaneously hypertensive rats. However, the underlying molecular mechanism remains unclear. OBJECTIVE： To investigate the effect of long-term aerobic exercise on endogenous formation of hydrogen sulfide in the kidney of spontaneously hypertensive rats. METHODS： Rat models of long-term aerobic exercise were established and randomly assigned to four groups： Wistar-Kyoto（WKY） rat static group, WKY rat exercise group, spontaneously hypertensive rat static group and spontaneously hypertensive rat exercise group. Moderate-intensity exercise on treadmill was given for 12 weeks. At 24 hours after model establishment, weight was weighted. Blood pressure was detected in the caudal artery. Blood and urine were collected for measuring biochemical indicators related to kidney functions. The degree of glomerular sclerosis was observed. Hydrogen sulfide production activity was detected in the kidney. RT-PCR was used to detect mRNA expression levels of hydrogen sulfide production-related enzymes. Simultaneously, oxidative stress of the kidney was observed. RESULTS AND CONCLUSION：（1） Long-term aerobic exercise obviously reduced body mass, systolic blood pressure and diastolic blood pressure, increased glomerular filtration rate and renal blood flow, decreased serum creatinine and urea nitrogen levels, urinary albumin levels, significantly reduced glomerular sclerosis score, increased hydrogen sulfide content in plasma and the rate of hydrogen sulfide formation in renal tissue, up-regulated cystathionine γ-lyase expression, obviously diminished malondialdehyde content in serum and kidney, and remarkably increased the reduced glutathione and oxidized glutathione ratio in spontaneously hypertensive rats.（2） Results indicated that long-term aerobic exercise could increase the generation of endogenous hydrogen sulfide in kidney, lessen oxidative stress in the kidney, and ameliorate renal dysfunction in spontaneously hypertensive rats.