AZ31镁合金表面Zn-Ca-P涂层的体外血液相容性

Hemocompatibility of zinc-calcium-phosphate coating on the surface of AZ31 magnesium alloy in vitro

背景:前期研究已在AZ31镁合金表面成功制备了Zn-Ca-P涂层,并研究了其抗腐蚀能力。目的:分析AZ31镁合金表面Zn-Ca-P涂层的血液相容性。方法:采用化学转化法在A Z31镁合金表面制备Zn-Ca-P涂层,采用扫描电镜观察涂层形貌,X射线衍射分析其成分,通过血小板黏附实验、动态凝血时间实验、溶血实验评价涂层的血液相容性。结果与结论:电镜观察显示,在A Z31镁合金表面形成一层花样或层状结构的涂层,其化学组成主要为不溶性磷酸盐Zn_3(PO_4)_2·4H_2O。AZ31镁合金Zn-Ca-P涂层表面黏附少量血小板,呈类圆形,无变形与伪足生成;动态凝血时间实验中,Z n-Ca-P涂层曲线经历时间长,呈缓慢向下倾斜形状,表现出更优的抗凝血性能;Z n-Ca-P涂层的溶血率低于5%。结果表明,AZ31镁合金表面Zn-Ca-P涂层具有良好的血液相容性。

BACKGROUND： In our previous studies, zinc-calcium-phosphate （Zn-Ca-P） coating has been successful y prepared on the surface of AZ31 magnesium al oy, which improves the corrosion resistance of the al oy. OBJECTIVE： To analyze the hemocompatibility of Zn-Ca-P coated AZ31 magnesium al oy in vitro. METHODS： Zn-Ca-P coating was prepared on the surface of AZ31 magnesium al oy using chemical transformation. Afterwards, the characteristics of the Zn-Ca-P coating were investigated using scanning electron microscope, and X-ray diffraction analyzed its components. Besides, hemocompatibility in vitro was evaluated by platelet adhesion assay, dynamic clotting time test and hemolysis test. RESULTS AND CONCLUSION： Under scanning electron microscope, a flower-like coating was formed ont surface of the AZ31 magnesium al oy, with the main chemical component of insoluble phosphates Zn3（PO4）2？4H2O. And some round distortionless blood platelets adhered to the surface of AZ31 magnesium al oy, with no pseudopodia. In dynamic clotting time test, the long curve of Zn-Ca-P coating tended to descend indicating better anticoagulant activity. And the hemolysis rate was below 5%. In conclusion, Zn-Ca-P coating has good hemocompatibility in vitro.