深圳市急性呼吸道感染儿童WU多瘤病毒感染检测及临床特征分析

Detection and clinical characterization of WU polyomavirus infection among pediatric patients with an acute respiratory tract infection in Shenzhen

目的了解深圳市急性呼吸道感染儿童WU多瘤病毒(WU polyomavirus,WUPoyV)感染特点及临床特征。方法收集深圳市两家医院急性呼吸道感染住院儿童咽拭子或鼻咽分泌物标本,采用PCR检测WUPoyV及9种常见呼吸道病毒,分析WUPoyV感染特点及临床表现。结果共收集2 025例患儿标本(年龄15d^14岁),10种病毒至少1种阳性971例(阳性率为47.95%)。检出WUPoyV 39例,阳性率1.93%,其中26例(66.67%)为单纯WUPoyV感染,13例(33.33%)为WUPoyV与其他病毒混合感染,感染者平均年龄23.3月,92.31%的患儿<4岁。WUPoyV感染全年均有发生,3~5月为发病高峰。WUPoyV感染症状主要表现为咳嗽、喘息、发热等,X线胸片检查主要为支气管肺炎或细支气管炎表现,临床症状和体征与其他呼吸道病毒感染相似;单纯感染与混合感染症状无明显差别。结论WUPoyV感染以<4岁儿童组常见,全年均可发生;春末夏初多见,其临床表现与普通的支气管肺炎和支气管炎类似,深圳市小儿呼吸道感染可能部分与WUPoyV有关。

Objective To ascertain the clinical and epidemiological characteristics of WU polyomavirus（WUPoyV）infection among pediatric patients with an acute respiratory tract infection in Shenzhen. Methods Nasopharyngeal aspirates were collected from pediatric patients with an acute respiratory infection at two tertiary hospitals.PCR was used to test for WUPoyV and 9other common respiratory viruses.The xTAGTM Respiratory Viral Panel Assay on the Luminex100 TMsystem was used to test specimens for the respiratory syncytial virus,influenza A virus,influenza B virus,parainfluenza virus（type 1and type 3）,human rhinovirus,adenovirus,and human metapneumovirus while monoplex PCR was used to test for human bocavirus and WUPoyV.The epidemiological characteristics and clinical manifestations of the viruses were compared and analyzed. Results A total of 2 025 specimens were obtained from 2 025 patients ranging in age from 15 days to 14 years of age over a 3-year period.Nine hundred and seventy-one patients（47.95%）tested positive for at least one viral pathogen.WUPoyV was detected in 39 pediatric patients at a rate of 1.93%.Twenty-six of those patients（66.67%）had a single infection and 13（33.33%）had multiple infections.The 39 patients infected with the WUPoyV had an average age of 23.3months and 36（92.31%）were younger than 4years of age.WUPoyV infections occurred year-round,with infections peaking from March to May.Chest X-rays mostly revealed bronchopneumonia and bronchiolitis in infected patients.The main clinical manifestations and signs in infected patients included coughing,shortness of breath,and a fever,and these manifestations and signs were similar to those of other viral infections.Differences in symptoms and signs in patients infected with WUPoyV alone and patients with a mixed infection that included WUPoyV were not statistically significant. Conclusion Children younger than 4years of age are more susceptible toWUPoyV.WUPoyV was detected throughout the year and peaked from late spring to early summer in Shenzhen.The clinical manifestations of a WUPoyV infection are bronchopneumonia and bronchiolitis,which are similar to the clinical manifestations of other common respiratory viruses.Acute respiratory tract infections in children in Shenzhen are somewhat correlated with WUPoyV.