藏紫菀总黄酮对模拟高原缺氧小鼠的保护作用

Protective Effect of Total Flavonoids from Aster Souliei Franch on Mice under Simulated High Altitude Hypoxia Environment

目的研究藏紫菀总黄酮对缺氧小鼠的保护作用。方法采用常压密闭缺氧耐受力实验和急性减压缺氧耐受力实验联合评价藏紫菀总黄酮的抗缺氧活性;采用低压氧舱模拟海拔8000 m高原环境,观察缺氧前后小鼠脑组织病理显微结构的改变,并测定脑组织中过氧化氢(H2O2)、丙二醛(MDA)、乳酸(LD)和乳酸脱氢酶(LDH)含量。结果藏紫菀总黄酮可以有效延长常压密闭缺氧小鼠的存活时间,降低急性减压缺氧小鼠的死亡率,且具有剂量依赖性关系(P<0.01);低压氧舱模拟海拔8000 m高原减压缺氧实验结果显示,与正常对照组相比,缺氧模型组小鼠脑组织出现病理性损伤,脑组织中MDA、H_2O_2、LD及LDH含量均显著升高(P<0.01);藏紫菀总黄酮组缺氧小鼠脑组织的病理损伤较缺氧模型组减轻,且脑组织中MDA、H_2O_2、LD及LDH含量均显著降低(P<0.01)。结论藏紫菀总黄酮能够对高原缺氧损伤小鼠起到保护作用。

Objective To study protective effect of total flavonoids from Aster souliei Franch on mice under simulated high altitude hypoxia environment. Methods The anti-hypoxia activity of total flavonoids from Aster souliei Franch was evaluated using normobaric hypoxia combined with acute decompression of hypoxia tolerance tests. Under simulated 8000 m altitude using low pressure oxygen cabin,the patho-microstructure changes of brain tissues were observed before and after anoxia,and contents of hydrogen peroxide（ H_2O_2）,malondialdehyde（ MDA）,lactic acid（ LD）and lactate dehydrogenase（ LDH） of brain tissues were detected. Results Total flavonoids from Aster souliei Franch effectively prolonged the survival time of mice under normobaric hypoxia environment. and reduced death rate of mice under acute decompression of hypoxia environment with dose dependent（ P〈0. 01）. The simulated 8000 m altitude examination showed that pathological damage was inflicted,and MDA,H_2O_2,LD and LDH contents of brain tissues were significantly increased in hypoxia groups,compared with those in control group（ P〈0. 01）. The pathological damage was moderate,and MDA,H_2O_2,LD and LDH contents of brain tissues were significantly decreased in total flavonoids from Aster souliei Franch group,compared with those in control group（ P〈0. 01）. Conclusion Total flavonoids from Aster souliei Franch can play a protective role on mice with high altitude hypoxia damage.