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A20对IL-17介导人牙周膜细胞RANKL表达影响 被引量：2

Effect of A20 on modulating RANKL expression of human periodontal ligament cells (hPDLCs) through IL-17
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摘要 目的探究A20对IL-17调控人牙周膜细胞表面RANKL表达的影响。方法转染人牙周膜细胞(h PDLCs)A20siRNA、慢病毒,获得A20沉默/过表达h PDLCs,分为沉默组、正常组、过表达组,IL-17(50 ng/ml)刺激一定时间,ELISA,PCR,Western-blot检测RANKL表达。结果 A20沉默组的h PDLCs RANKL蛋白表达水平较正常对照组高,A20过表达组较正常对照组低,IL-17(50ng/ml)刺激下,A20沉默组较正常对照组RANKL表达水平高,过表达组较正常对照组低。结论本研究首次发现A20可能参与调控IL-17引起的h PDLCs RANKL表达。 Objective To investigate the potential function of A20 on modulating RANKL expression of the human periodontal liga- ment cells （hPDLCs） regulated by IL-17. Methods HPDLCs were transfected of A20siRNA and slow virus, and then hPDLCs by suppression/over-expression of A20 were obtained, which were divided into suppression group, control group and over-expression group. Treated with IL-17 （50 ng/ml） for certain time, protein and mRNA levels of RANKL were determined by enzyme-linked immu- nosorbent assays （ELISA）, real-time PCR （RT-PCR） and western blotting. Results Suppression/over-expression of A20 in hPDLCs increased/decreased the expression of RANKL protein compared to the control group. Upon the stimulation of IL-17 （50ng/ml）, simi- lar results were observed in the three groups of hPDLCs. Conclusion Our study revealed for the first time that IL-17 induced RANKL expression of hPDLCs may be interfered by A20.
作者 叶宇 俞文伟 陈旭 茅飞飞 徐艳 李璐
机构地区 南京医科大学口腔疾病研究江苏省重点实验室 南京医科大学附属口腔医院牙周科
出处 《口腔医学》 CAS 2016年第6期489-493,共5页 Stomatology
关键词 牙周膜成纤维细胞 白介素17 肿瘤坏死因子α诱导蛋白 细胞核因子κB受体活化因子配体 periodontal ligament cells IL-17 tumor necrosis factor alpha-induced protein3 RANKL ]
分类号 R780.2 [医药卫生—口腔医学]
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参考文献19

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二级参考文献16

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口腔医学
2016年 第6期

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