牙周组织中β-防御素与TOLL样受体表达水平的研究

Study on the expression of human beta defensins and TOLL like receptors in the periodontal tissues

目的研究人β-防御素(h BDs)与Toll样受(TLRs)体这两大类免疫物质在牙周组织表达以及由于慢性牙周炎引起的表达水平变化。方法在口腔科门诊征集23例慢性牙周炎患者和15例健康对照者,分别在其进行牙周手术和拔牙手术时收集牙周组织,通过实时定量PCR和免疫组化实验分析h BDs和TLRs的表达水平变化。结果作为牙周炎相关的免疫因子,h BDs和TLRs在健康人和慢性牙周炎患者的牙周组织中的表达水平均有变化,但变化趋势有所不同。结论防御素和TLRs在牙周组织中的表达水平因为牙周炎的存在而发生一定的变化。由于表达部位不同,提示在慢性牙周炎进程中,这两大类内源性免疫因子之间也存在相互作用。

Objective To study the expression of human Bata Defensins （hBDs） and Toll like receptors （TLRs） in periodontal tis- sues and the changes of the expression level in chronic periodontitis. Methods 23 chronic periodontitis patients and 15 healthy con- trols were selected in the department of stomatology. The periodontal tissues were collected in the patients with chronic periodontitis and healthy controls. The expression levels of TLRs and hBDs were analyzed by real-time quantitative PCR and immunohistoehemistry. Re- suits The expression levels of TLRs and hBDs in the periodontal tissues of healthy individuals and patients with chronic periodontitis were changed, but the changing trend was different. Conclusion The expression levels of hBDs and TLRs in the periodontal tissues were analyzed in this study. According to the research results, the expression level of these two kinds of endogenous immune factors in the periodontal tissues may change with the presence of periodontitis. Because of the different expression sites, it is suggested that there are interactions between these two endogenous immune factors in the process of chronic periodontitis.