油菜田看麦娘对精喹禾灵抗性监测及与烯草酮交互抗性

Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field

通过对看麦娘抗药性水平的检测以及抗性机理的研究,以期为油菜田看麦娘的抗性治理和科学合理用药提供理论依据。采用整株法测定了长江中下游地区的4个省13个市17个地区的油菜田看麦娘生物型对精喹禾灵的抗性水平及与烯草酮的交互抗性,分别测定了精喹禾灵与烯草酮对抗性看麦娘（R-TY）、敏感看麦娘（S-NJ）体内GSTS活性。抗精喹禾灵监测结果显示：TY生物型相对抗性倍数最高,为28.92,GR50为90.183。其次为YZ生物型和BLD生物型,GR50分别为34.480和45.472,相对抗性倍数分别为11.06和14.58。其余生物型相对抗性倍数在1.62~9.16之间。与烯草酮的交互抗性结果显示：对精喹禾灵产生抗性的TY、BLD生物型对烯草酮也产生了抗性,存在交互抗性,而对精喹禾灵敏感的DYX生物型看麦娘却对烯草酮产生了抗性,说明看麦娘生物型中存在不同的抗性模式。GSTS活性测定结果显示：经药剂处理后,R-TY生物型GSTS活性始终比敏感生物型S-NJ高,表明TY生物型产生抗性的原因可能是GSTS对药剂的代谢作用增强。

By detecting the resistance level of Alopecurus aequalis Sobol., and studying its resistancemechanism, a theoretical basis for rational drug use can be provided for A.aequalis resistance management inrape field. The resistance levels of Alopecurus aequalis Sobol. biotypes to quizalofop-P-ethyl and the cross-resistance with clethodim in 17 areas in the middle and lower reaches of Yangtze River were determined usingthe whole-plant assay method. The glutathione-s-transferase（GSTS） enzyme activity was studied in resistantbiotype（R-TY） and susceptible biotype（S-NJ） with quizalofop-P-ethyl and clethodim. The anti-quizalofopmonitoring results showed that： TY biotype had the highest relative resistance multiple, which was 28.92, GR50 was 90.183. And then were YZ and BLD biotype, of which, GR50 was 34.480 and 45.472, respectively, and therelative resistance multiple was 11.06 and 14.58, respectively. The relative resistance multiples of otherbiotypes were ranged from 1.62 to 9.16. The cross-resistance with clethodim showed that there was a cross-resistance between quizalofop and clethodim in TY and BLD biotype, while DYX, the quizalofop sensitivebiotype, was resistant to clethodim, indicating A. aequalis biotypes might have other resistance patterns. GSTSactivity assay showed that： after drug treatment, the GSTS activity in R-TY biotype was always higher than that in the sensitive biotype S-NJ, indicating that TY biotype＇s resistance to drug might be due to the GSTSenhancing effect on drug metabolism.