鸡肠道定殖乳酸杆菌分离鉴定及药物敏感性分析

Separation and identification of colonized Lactobacillus from chickens intestine and analyzation antibiotics susceptibility

为筛选分析鸡肠道内定殖乳酸杆菌及其对抗生素药物敏感性,选取健康鸡肠道内容物,利用MRSCa CO3培养基平板,42℃厌氧培养,选择革兰氏阳性、不运动、无芽孢、杆状细菌检测过氧化氢酶、氧化酶和硝酸盐还原活性,糖发酵反应管作生化反应表型鉴定,利用PCR扩增16S r RNA并测定序列。结果表明,获得8株乳酸杆菌,其中包括3株Lactobacillus crispatus、2株Lactobacillus johnsonii、2株Lactobacillus salivarius和1株Lactobacillus saerimneri。8株乳酸杆菌在37和42℃均生长良好;抑菌试验表明,对革兰氏阳性及阴性细菌均有一定抑菌活性。体外粘附试验表明,8株乳酸杆菌均具有一定粘附特性,可在肠道内定殖。抗生素敏感性试验表明,8株乳酸杆菌对多种抗生素敏感。

In order to select colonized Lactobacil us from chickens intestine and analyzation antibiotics susceptibility, Lactobacil us were isolated from the intestine of healthy chickens. After using MRS-CaCO3 plates, anaerobic culturing in 42 ℃, bacterias those are Gram-positive, stil , no spores and rod-like were tested by oxidase, catalase reaction, and biochemical reactions. Eight strains with different phenotype were carried 16S rRNA sequence analysis by using PCR, and the results showed that three of the eight strains were Lactobacil us crispatus, two strains were Lactobacil us johnsoni , two strains were Lactobacil us salivarius and one strain were Lactobacil us saerimneri. Eight strains were able to grow wel in both 37 and 42 ℃. And al the strains exhibited sensitivity to various antibiotics, inhibited the growth of both Gram-positive and Gram-negative bacteria. And the in vitro adherence test showed that al the isolates had the ability to adhere the guts. Antibiotics susceptibility test showed that eight strains were sensitive to many antibitics.