乳腺癌细胞中miR-1246、ZO-1、vimentin的表达及相关性研究

Expression and correlation of miR-1246,ZO-1 and vimentin in breast cancer cells

目的检测miR-1246、ZO-1、vimentin在乳腺正常上皮细胞MCF10A和乳腺癌癌细胞MCF7、MDA-MB-231中的表达,并探讨miR-1246在促进乳腺癌上皮-间质化(epithelial-mesenchymal transition,EMT)中的作用。方法用荧光定量PCR法检测乳腺正常上皮细胞MCF10A、乳腺癌细胞MCF7、MDA-MB-231中miR-1246的相对表达量,用免疫印迹法检测乳腺正常上皮细胞MCF10A、乳腺癌细胞MCF7、MDA-MB-231中紧密连接蛋白ZO-1、波形蛋白vimentin的表达情况。采用SPSS 16.0统计软件分析,用U检验、t检验和Spearman相关分析分析数据。结果miR-1246和ZO-1在恶性乳腺癌细胞中的表达明显降低(P<0.05),vimentin在恶性乳腺癌细胞中的表达明显增加(P<0.05),miR-1246与ZO-1表达呈正相关(r=0.940,P<0.05),miR-1246与vimentin表达呈负相关(r=-0.714,P<0.05)。结论 miR-1246表达水平与EMT标志物ZO-1、vimentin的表达水平存在一定的相关性,提示miR-1246在促进乳腺癌EMT过程中扮演重要角色。

Objective To investigate the expression and correlation of ZO-1,vimentin and miR-1246 in normal breast epithelial cells（ MCF10A） and breast cancer cells（ MCF7,MDA-MB-231）,and analyze the role of miR-1246 in promoting breast cancer epithelial-mesenchymal transition（ EMT）. Methods The relative expression levels of miR-1246 in MCF10 A,MCF7 and MDA-MB-231 were detected by q PCR assay; the expression of ZO-1 and vimentin protein in MCF10 A,MCF7 and MDA-MB-231 were detected by WB assay. Data were analyzed using U test,t test and spearman rank correlation analysis by SPSS 16. 0. Results The expression of miR-1246 and ZO-1 in malignant breast cancer cells were significantly decreased（ P〈0. 05）,the expression of vimentin in malignant breast cancer cells was significantly increased（ P〈0. 05）. MiR-1246 was positively correlated with ZO-1 expression（ r = 0. 940,P〈0. 05）,miR-1246 and vimentin expression were negatively correlated（ r =- 0. 714,P〈0. 05）. Conclusion The expression level of miR-1246 and EMT markers is correlated,so miR-1246 is suggested to play an important role in the promotion of EMT in breast cancer.