绿海龟健康幼体心肝肾功能血液生化指标参考值研究

Research on the plasma biochemistry of cardiac function,liver function and renal function from healthy larval green turtles( Chelonia mydas ) in captivity

采集了39只人工养殖的健康幼体绿海龟（1.5-2.5龄）、6只健康成体绿海龟（10-20龄）和3只野生成体绿海龟,用Beckman AU5800和TNI BECKMAN Access 2测定和分析了反映绿海龟心肝肾功能的17项血液生化指标,结果发现野生个体的生化指标与人工养殖的绿海龟差异较大,谷草转氨酶（P=0.002）、白蛋白（P=0.015）、碱性磷酸酶（P=0.010）、谷丙转氨酶（P=0.010）等均有明显差异,人工养殖绿海龟除碱性磷酸酶与年龄呈负相关外没有明显差异,不同性别的个体在生化指标上差异不显著。本实验结果与野生绿海龟各研究结果对比发现,野生个体的指标差异非常显著,难以作为健康指标的参考。本研究建立了人工绿海龟的心肝肾功能血液生化指标参考值,为野生绿海龟血液生化研究提供参考,为指导绿海龟养殖过程中的疾病早期诊断和预防提供依据。

The paper detected 17 biochemistry indices of the 39 healthy captive hatching larval green turtles（ 1. 5- 2. 5 years old）,six healthy adult green turtles（ 10- 20 years old） and 3 wild turtles using the Beckman AU5800 and TNI BECKMAN Access 2 to analyze. The results showed that there was large difference between wild and captive ones on aspartate aminotransferase（ P = 0. 002）,albumin（ P = 0. 015）,alkaline phosphatase（ P = 0. 010）,glutamic pyruvic transaminase（ P = 0. 010）,etc. The biochemistry indexes presented no difference in captive green turtles between different ages and different sexes except for alkaline phosphatase. Alkaline phosphatase and age showed a negative correlation. Compared with the research results of others＇ and the experiment＇s wild green turtles,the indexes of wild individuals had great difference which could not be regarded as the reference for health indicators. This study established blood biochemistry reference values of captive green turtles in cardiac,liver and renal functions. This provides a reference for the wild green turtles＇ blood biochemistry research and established a basis for the early diagnosis and disease prevention of captive green turtles.