摘要
以新疆野扁桃(Prunus tenella)花粉为试材,构建其自交不亲和性相关基因PetSSK1的植物表达载体pCAMBIA1303-PetSSK1,采用根癌农杆菌介导法,将该基因导入矮牵牛,并研究了不同种类及浓度抗生素对矮牵牛再生的影响。结果表明:该试验成功获得了转化新疆野扁桃自交不亲和相关基因PetSSK1的矮牵牛植株,并筛选出矮牵牛的卡那霉素(Kan)和头孢霉素(Cef)的敏感浓度分别为25、300mg·L^(-1),并进一步通过PCR分子检测确认其为转基因阳性植株。
Taking the pollen of Xinjiang wild almond（Prunus tenella）as material,the expression plasmid pCAMBIA1303-PetSSK1 with self-incompatibility related gene PetSSK1 was constructed.Then it was transferred into Petunia mediated by Agrobacterium tumefaciens,and the effect of different kinds and concentration of antibiotics on Petuniaregeneration was studied.The results showed that the Petuniataking self-incompatibility related gene PetSSK1 of Xinjiang wild almond was gotten successfully.The sensitive concentration of Kan and Cef were 25 mg·L^-1 and 300 mg·L^-1,respectively.And the Petunia were further confirmed as positive plants by PCR detection.
出处
《北方园艺》
CAS
北大核心
2016年第12期96-100,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(31260465)
国家林业公益性行业科研专项资助项目(201304701-1)
新疆维吾尔自治区科技计划资助项目(201130102-1-5)
新疆维吾尔自治区果树学重点学科资助项目(201007)
关键词
野扁桃
PetSSK1
植物表达载体
根癌农杆菌介导
矮牵牛
wild almond（Prunus tenella）
PetSSK1
plant expression vector
Agrobacterium tumefaciens
Petunia hybrida
