不同激素配比对白及愈伤组织诱导、增殖和分化的影响

Effect of Different Hormone Combinations on the Induction,Proliferation and Differentiation of Callus in Bletilla striata

以白及为试材,以MS和1/2MS为基本培养基,研究了愈伤组织诱导、增殖和分化培养基中的最佳激素种类和浓度配比。结果表明:在MS中添加6-BA 1 mg·L^(-1)和2,4-D3mg·L^(-1)时白及愈伤的诱导率最高,达89.89%。2,4-D是诱导白及愈伤生成的关键激素,而在基本培养基中单添加6-BA时并不能诱导愈伤组织的生成,同时添加2,4-D和6-BA能有效促进愈伤的诱导,且愈伤诱导率随着2,4-D浓度的增加而逐渐增加,但优质愈伤率也随之降低。添加6-BA 0.1mg·L^(-1)和2,4-D 2mg·L^(-1)的增殖培养基中愈伤细胞的增殖率最高,30d的增殖倍数达7.457。在分化培养基中添加NAA和TDZ有利于白及愈伤组织分化,分化率随着二者浓度的增加而增加,且NAA的促分化效果优于TDZ。MS+2,4-D 2mg·L^(-1)+NAA 1mg·L^(-1)为最佳分化培养基,分化30d时其分化率为86.67%。

Bletilla striata（Thunb.）Rchb.f.was used in this study to screen the optimal mediums in callus induction,proliferation and differentiation through the comparisons of different hormones combined with the concentrations based on the medium of MS and 1/2MS.The results showed that the highest inducing rate was observed as 89.89% at the combination of MS＋6-BA 1mg·L^-1＋2,4-D 3mg·L^-1.6-BA（6-Benzylaminopurine）alone in the basic medium exhibited disability in producing the callus,while this disability was determined by adding 2,4-D（2,4-D ichlorophenoxyacetic acid）simultaneously.And the inducing rate increased as more 2,4-D was added,while the products quality decreased as the increasing of 2,4-D in the basic medium.Additionally,the combination of MS＋6-BA0.1mg·L^-1＋2,4-D 2mg·L^-1 exhibited the highest proliferation rate（745.7%）than the other combinations after30 days incubation.Increasing callus differentiation rate was observed as the increasing of NAA（alpha-Naphthylacetic acid）and TDZ（thidiazuron）in the basic medium,while NAA alone possessed better effect than TDZ.And the combination of MS＋2,4-D 2mg·L^-1＋NAA 1mg·L^-1 exhibited the highest differentiation rate（86.67%）than other combinations after 30 days differentiation.