替米沙坦对小鼠巨噬细胞M1/M2亚型极化的影响

Effect of Telmisartan on mice macrophage M1/M2 polarization

目的探讨替米沙坦对小鼠巨噬细胞M1/M2亚型极化的影响。方法分别用脂多糖(LPS)联合干扰素-γ(IFN-γ)和白介素-4(IL-4)诱导小鼠巨噬细胞M1/M2型极化,用免疫荧光法检测极化结果。在M1型巨噬细胞中分别加入0.1、1、10μmol/L替米沙坦,同时设立空白对照组及溶剂对照组,Western blot法检测各组巨噬细胞亚型标志物诱导性一氧化氮合酶(iN OS)和精氨酸酶Ⅰ(ArgⅠ)的表达情况,ELISA法检测各组培养液上清中IL-6、IL-10表达情况。结果在LPS+IFN-γ诱导的小鼠巨噬细胞中M1型巨噬细胞标志物iN OS、IL-6的表达水平明显升高,替米沙坦干预后,各干预组M1型巨噬细胞标志物iN OS、IL-6的表达水平下降(P<0.05),随着替米沙坦浓度的增加而降低,而代表M2型巨噬细胞标志物的ArgⅠ和IL-10表达水平上升(P<0.05)。结论替米沙坦可以抑制LPS+IFN-γ诱导的小鼠巨噬细胞M1型极化,促进M1型巨噬细胞向M2型巨噬细胞转化。

Objective To investigate the effect of Telmisartan on the M1/M2 polarization of mice macrophage.Methods Mice macrophage was induced to M1/M2 polarization by LPS＋IFN-γ and IL-4 respectively,and tested by immunofluorescence. Meanwhile cells were treated with 0. 1,1,10 μmol / L Telmisartan,blank control group and vehicle control group were established at the same time. The biomarkers i NOS and Arg Ⅰ were tested by Western blot,and the cytokines IL-6 and IL-10 were tested by ELISA assay. Results The biomarker i NOS and IL-6,secreted by M1 macrophage,were apparently increased in the macrophages induced by LPS ＋ IFN-γ. However,after being treated with Telmisartan,the expressions of i NOS and IL-6 were obviously decreased（ P〈0. 05）,as the concentration higher the expression lower. While,the expression of Arg I and IL-10,which represented the M2 macrophage,increased（ P〈0. 05）. Conclusion Telmisartan can inhibit the M1 polarization of mice macrophage RAW264. 7 induced by LPS and IFN-γ and transform M1 macrophage polarization to M2 macrophage polarization.