PCR-RDB技术检测中国籍OSCC及OLK患者中的HPV

Detection of human papillomavirus in Chinese patients with oral squamous cell carcinoma and oral leukoplakia by PCR-RDB method

目的:研究口腔鳞癌(OSCC)及口腔白斑(OLK)中国患者中的人乳头瘤病毒(Human Papillomavirus,HPV)感染率。方法:提取35例OSCC及35例OLK患者病损组织DNA,通过聚合酶链式反应结合反向点杂交技术(Polymerase Chain Reaction-Reverse Dot Blot Hybridization,PCR-RDB)检测23型HPV DNA,包括HPV6,11,16,18,31,33,35,39,42,43,45,51,52,53,56,58,59,66,68,73,81,82和83。结果:35例OSCC及35例OLK患者中HPV DNA均为阴性。结论:HPV感染在中国的OSCC及OLK患者中非常见分子事件。

Objective：To detect human papillomavirus（HPV） in oral squamous cell carcinoma（OSCC） and oral leukoplakia（OLK） of Chinese patients. Method：We extracted 35 OSCC and 35 OLK tissue DNA samples. PCR-RDB assay was performed to detect 23 subtypes of HPV,which including HPV6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 45, 51, 52, 53, 56, 58,59, 66, 68, 73, 81, 82 and 83. Result：All of the 35 OSCC and 35 OLK patients were negative for HPV DNA. Conclusion：HPV infection was not a frequent molecular event in OSCCs and OLKs of Chinese patients.