RT-PCR检测SALL4在卵巢生殖细胞肿瘤中的表达

Expression and clinical significance of SALL4 by RT-PCR

目的研究SALL4与卵巢生殖细胞肿瘤的关系,探讨卵巢生殖细胞肿瘤的发生机制及相关基因的功能,寻找卵巢生殖细胞肿瘤早期检测、诊断的新指标。方法采用逆转录聚合酶链反应(RT-PCR)检测40例卵巢生殖细胞肿瘤、40例卵巢非生殖细胞肿瘤中SALL4的表达,分析SALL4与卵巢生殖细胞肿瘤的关系。结果 SALL4在正常卵巢组织中均无表达,在卵巢生殖细胞肿瘤中的阳性表达率90.00﹪(36/40)显著高于卵巢非生殖细胞肿瘤17.50﹪(7/40),两者比较差异有统计学意义(P﹤0.01)。结论 SALL4在正常卵巢组织中无表达,在卵巢非生殖细胞肿瘤中极低表达,而在卵巢生殖细胞肿瘤中高表达,提示SALL4可能是一种新的卵巢生殖细胞肿瘤早期检测、评估恶性程度及预后的肿瘤标记物。

Objective：To study the relationship between theexpression of SALL4 and human ovarian germ cell tumors in order to find out the pathogenesis of carcinogengsis and the role of the cancer associated genes in the level of geng and find out a new index for early diagnosis and prognosis detection of ovarian germ cell tumors. Methods： Reverse Polymerase Chain Reaction（RT-PCR） method was employed to detect the expression of SALL4 in human germ cell tumors. Results： The positive expression rate of SALL4 in normal ovarian tissues was zero. The positive expression rate of SALL4 in germ cell tumors was 90. 00% （36/40） , which was higher than that in non-germ cell tumor＇s 17.50% （7/40） （P 〈0. 01 ）. Conclusion ： The expression rate of SALL4 in normal ovarian tissues is zero and in germ cell tumors is low, but the expression of SALL4 in germ cell tumors is higfier. So SALL4 maybe a new ovarian germ ceil tumors markers that can predict the prognosis of the patients.