蓝氏贾第鞭毛虫抗原快速检测与多重PCR检测的比较

Comparison between Giardia lamblia antigen screening test and multiplex PCR assay

目的比较蓝氏贾第鞭毛虫抗原快速检测法与多重PCR检测法检测结果间的相关性,为蓝氏贾第鞭毛虫快速筛查提供依据。方法采用抗原快速检测法与多重PCR检测法同时测定102例具有相关症状体征病人的粪便标本,确定病人蓝氏贾第鞭毛虫感染状况。结果共检测腹泻患者粪便标本102例,抗原快速检测法检测阳性81例,阴性21例,多重PCR检测阳性标本83例,阴性19例。抗原快速检测阳性而多重PCR检测确认阴性的标本0例;抗原快速检测阴性而多重PCR检测阳性的标本2例。抗原快速检测对蓝氏贾第鞭毛虫的阳性预示值为100.0%,阴性预示值为90.5%,诊断灵敏度是97.6%,诊断特异性是100.0%。结论采用抗原快速检测法检测蓝氏贾第鞭毛虫抗原,15 min内可筛查标本中的病原体,利于疾病的早期预防、诊断和治疗,防止社区/区域流行。

Objective To explore the correlation between Giardia lamblia antigen rapid screening test and multiple PCRassay in the detection of Giardia lamblia antigen,in order to provide the evidence for clinical diagnosis. Methods The stoolspecimens from 102 patients with diarrhea were collected and detected for G. lamblia antigen by using the rapid screening testand multiple PCR assay. Results From January 2011 to December 2015,102 stool specimens were detected by the rapidscreening test and multiple PCR. Among the 102 specimens,81 were positive and 21 were negative by the rapid antigenscreening test,and 83 were positive and 19 were negative by multiple PCR. The number of samples of rapid screening testpositive but multiple PCR assay negative was 0,and the number of samples of rapid screening test negative but multiple PCRassay positive was 2. The positive predictive value to G. lamblia of the rapid screening test was 100.0%,and the negativepredictive value was 90.5%. The sensitivity of the rapid screening test was 97.6% and specificity was 100.0%. In addition,thetime of rapid screen test for Giardia antigen detection was within 15 minutes. Conclusion The Giardia antigen rapid screentest is effective and fast,which helps early treatment and prevention from community or area endemics.