齿叶黄皮中香豆素类化学成分研究

Studies on the Coumarins from Stems and Leaves of Clausena Dunniana

综合运用硅胶柱层析、ODS柱层析、Sephadex LH-20凝胶柱层析以及制备型HPLC等现代色谱分离技术,对芸香科黄皮属植物齿叶黄皮(Clausena dunniana)枝叶的化学成分进行系统分离与纯化。结合理化性质和多种现代波谱鉴定技术,并通过与文献报道的数据对照,鉴定了从齿叶黄皮枝叶90%乙醇提取物的石油醚萃取部位中分离得到8个香豆素类化合物的化学结构,分别为6-羟基-8-甲氧基-香豆素(1)、秦皮素(2)、异秦皮素(3)、佛手柑内酯(4)、花椒毒素(5)、异欧前胡素(6)、九里香酮(7)和蛇床子素(8)。以上所有化合物均为首次从齿叶黄皮中分离得到。

The chemical consituents from stems and leaves of Clausena dunniana were separated and purified by column chromatographies with silica gel, ODS Sephadex LH-20 and Pre-HPLC. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic analysis, as well as comparisons with the data in literature. Eight compounds were isolated and elucidated as 6-hydroxy-8-methoxy-coumarin（I）, fraxidin（2）, isofraxidin（3）, bergapten（4）, xanthotoxin（5）, imperatorin（6）, murrayone（7）, and osthol（8）. All compounds were isolated from C. dunniana for the first time.