半仿生-回流法提取金线莲中黄酮的研究

Extraction of Flavones from Anoectochilus Roxburghii(wall) Lindl using Semi Bionic-Reflux Method

文章采用半仿生-回流提取技术,以不同p H的乙醇-水溶液作为提取溶剂,分别进行单因素筛选和正交实验的方法,考察了p H,溶液组成和回流时间对金线莲中黄酮提取量的影响,优化了金线莲中提取的工艺条件。结果表明,半仿生-回流技术提取金线莲中黄酮的最佳条件:对于0.5克金线莲,提取液为200 m L p H=4的50%乙醇溶液,回流时间为2.0 h,黄酮提取量达到30.21 mg/g。

The extraction of flavones from anoectochilus roxburghii （wall） lindl was studied by semi bionic-reflux methods. The effects ofpH, ethanol content in solvent, reflux＇s volume and reflux＇s time on extraction of flavones were optimized by Single factor screening and L：6（43） orthogonal design test, respectively. The results indicated that the extracting amount using pH=4 50 % ethanol solutions as a solvent are better than those by ethanol as a solvent. The optimum conditions for extracting flavones by semi bionic-reflux method are pH-4, solvent volume 200 mL （50 % ethanol solutions）, reflux＇s time 2.5 h. Under the optimum conditions, the extracting amount flavones reach 30.21 mg in 1 g of anoectochilus roxburghii （wall.） Lindl.