急性痛风性关节炎患者血清IL-1β、IL-6、IL-18及TNF-α水平的研究

Research of Serum Level of IL-1β, IL-6, IL-18 and TNF-α of Patients with Acute Gouty Arthritis

目的探讨急性痛风性关节炎(acute gouty arthritis,AGA)患者血清白细胞介素(interleukin,IL)-1β、IL-6、IL-18及肿瘤坏死因子(tumor necrosis factor,TNF)-α水平的变化及意义。方法用ELISA法测定100例AGA患者(观察组,T)和100名健康查体者(对照组,C)血清IL-1β、IL-6、IL-18及TNF-α水平。观察组与对照组比较采用独立样本t检验,一般生理指标及血生化指标用独立样本t检验,计数资料采用χ2检验。计量资料用(x-±s)表示。采用IBM SPSS 19.0软件包对所有数据进行统计学分析。结果观察组与对照组相比,IL-1β、IL-6、IL-18及TNF-α水平均显著升高,差异有统计学意义(P<0.01)。结论 IL-1β、IL-6、IL-18及TNF-α水平在AGA发病时升高,提示它们参与了炎性反应过程,在炎症机制中发挥了重要作用。

Objective To investigate the change of serum level of interleukin（IL）-1β, IL-6, IL-18 and tumor necrosis factor（TNF）-α in the subjects with acute gouty arthritis（AGA） and their significance. Methods Based on standards of American College of Rheumatology（ACR） in 1997, 100 AGA patients were selected as AGA group（T） and 100 healthy subjects as control group（C）. IL-1β, IL-6, IL-18 and TNF-α were measured using Enzyme-linked immunosorbent assay（ELISA） in the two groups. The statistical data were analyzed by IBM SPSS 19.0 software. Independent-samples t test was used in two groups. Result The expression of IL-1β, IL-6, IL-18 and TNF-α increased significantly when compared to C（P〈0.01）. Conclusion Increased expression of IL-1β, IL-6, IL-18 and TNF-α level are involved in the inflammatory response and play important roles in the pathogenesis of the process of AGA.