苹果K^+转运蛋白基因家族鉴定及表达分析

Identification and Expression Analysis of Potassium Transporter Gene Family in Apple

利用苹果基因组筛选K^+转运蛋白基因,分析其系统发育关系,通过q RT-PCR检测它们在平邑甜茶各器官组织和不同发育阶段果实的表达特征。结果表明,在苹果中存在65个K^+转运蛋白基因,包括CHX家族(33个)、HAK家族(24个)、HKT家族(1个)和KEA家族(7个),它们与拟南芥K^+转运蛋白基因高度同源,其基因结构相对保守,并且不均匀地分布在13条染色体上。定量表达分析发现,K^+转运蛋白基因具有不同的表达模式,其中在根中高度表达的32个,在叶片中高度表达的12个,在茎尖中高度表达的11个,在果实中高度表达的19个。研究结果为揭示苹果K^+转运蛋白基因的功能提供了基础资料。

In this study,the information that contained phylogenetic relationship and distribution of apple potassium（K＋）transporter genes was analyzed based on Malus genome database,and their expression characteristics were explored by means of q RT-PCRs in apple. The results showed that 65 K＋transporter genes were identified,and they were divided into 4 families：CHX family,HAK family,HKT family and KEA family,including 33,24,1 and 7 members,respectively. These genes are highly homologous with Arabidopsis thaliana K＋ transporter genes. Chromosomal localization exhibited that these K＋ transporter genes were distributed in the thirteen of seventeen apple chromosomes unevenly. Quantitative expression analysis showed that most of the K＋ transporter genes had differential expression patterns. The thirty-two genes had higher expression abundance in root while 12 genes in mature leaves,11 genes in shoot tips and 19 genes in fruit. The results lay a basis for revealing the function of K＋ transporter genes in apple.